Manganese in PDB 5cnq: Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions

The structure of Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions, PDB code: 5cnq was solved by Y.J.Liu, A.D.J.Freeman, A.C.Declais, T.J.Wilson, A.Gartner, D.M.J.Lilley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	49.29 / 2.60
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	98.582, 98.582, 119.619, 90.00, 90.00, 120.00
R / Rfree (%)	21.2 / 25.2

The binding sites of Manganese atom in the Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions (pdb code 5cnq). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions, PDB code: 5cnq:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:82.4 occ:1.00 OE1 A:GLU122 2.2 76.0 1.0 O A:HOH630 2.3 64.8 1.0 O A:HOH608 2.6 75.8 1.0 O A:HOH653 2.9 79.1 1.0 CD A:GLU122 3.0 70.8 1.0 OE2 A:GLU122 3.1 78.2 1.0 O H:HOH101 3.8 67.4 1.0 O A:HOH611 3.9 89.9 1.0 OE1 A:GLU140 4.0 84.1 1.0 OD2 A:ASP38 4.0 66.3 1.0 OD1 A:ASP79 4.2 67.2 1.0 OD2 A:ASP79 4.3 74.6 1.0 CG A:GLU122 4.3 60.4 1.0 O A:HOH631 4.5 56.8 1.0 CB A:ALA40 4.6 46.5 1.0 OD1 A:ASP38 4.6 68.1 1.0 O A:HOH609 4.6 71.3 1.0 CB A:GLU122 4.7 55.2 1.0 N A:GLU122 4.7 44.6 1.0 CG A:ASP79 4.7 67.0 1.0 OE2 A:GLU120 4.7 94.7 1.0 N A:ALA121 4.7 48.8 1.0 CG A:ASP38 4.8 63.5 1.0 CB A:GLU120 4.8 56.4 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Holliday Junction-Resolving Enzyme GEN1 (Wt) in Complex with Product Dna, MG2+ and MN2+ Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn502 b:90.6 occ:1.00 O A:HOH604 1.8 68.3 1.0 O A:HOH611 2.0 89.9 1.0 OD2 A:ASP143 2.2 73.8 1.0 OD2 A:ASP141 2.3 66.4 1.0 O A:HOH631 2.8 56.8 1.0 O H:HOH101 3.0 67.4 1.0 CG A:ASP141 3.1 65.3 1.0 OD1 A:ASP141 3.1 63.6 1.0 CG A:ASP143 3.2 70.3 1.0 OP1 H:DG17 3.4 0.3 1.0 CB A:ASP143 3.8 61.0 1.0 O5' H:DG17 3.9 99.5 1.0 OD1 A:ASP143 4.1 72.1 1.0 P H:DG17 4.2 0.1 1.0 O A:HOH608 4.3 75.8 1.0 OE2 A:GLU122 4.4 78.2 1.0 O A:GLY2 4.5 98.8 1.0 CB A:ASP141 4.5 65.4 1.0 OE1 A:GLU140 4.6 84.1 1.0 OP2 H:DG17 4.8 0.7 1.0 CB A:GLU140 4.9 73.4 1.0

Y.Liu, A.D.Freeman, A.C.Declais, T.J.Wilson, A.Gartner, D.M.Lilley. Crystal Structure of A Eukaryotic GEN1 Resolving Enzyme Bound to Dna. Cell Rep V. 13 2565 2015.
ISSN: ESSN 2211-1247
PubMed: 26686639
DOI: 10.1016/J.CELREP.2015.11.042