Manganese in PDB 4zb5: A Form of Glucose Isomerase Collected at 100K.

All present enzymatic activity of A Form of Glucose Isomerase Collected at 100K.:
5.3.1.5;

The structure of A Form of Glucose Isomerase Collected at 100K., PDB code: 4zb5 was solved by J.Sandy, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	68.90 / 2.00
Space group	I 2 2 2
Cell size a, b, c (Å), α, β, γ (°)	92.904, 98.152, 102.704, 90.00, 90.00, 90.00
R / Rfree (%)	11.5 / 15.4

The binding sites of Manganese atom in the A Form of Glucose Isomerase Collected at 100K. (pdb code 4zb5). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the A Form of Glucose Isomerase Collected at 100K., PDB code: 4zb5:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the A Form of Glucose Isomerase Collected at 100K.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of A Form of Glucose Isomerase Collected at 100K. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:6.4 occ:1.00 O4 A:GLC403 2.0 6.0 1.0 OD2 A:ASP287 2.1 5.0 1.0 OE2 A:GLU181 2.1 5.0 1.0 OD2 A:ASP245 2.2 3.9 1.0 OE1 A:GLU217 2.2 3.4 1.0 O3 A:GLC403 2.2 6.2 1.0 C4 A:GLC403 3.0 6.3 1.0 C3 A:GLC403 3.0 6.1 1.0 CD A:GLU181 3.1 4.6 1.0 CG A:ASP287 3.2 4.8 1.0 CD A:GLU217 3.3 3.5 1.0 OE1 A:GLU181 3.3 4.8 1.0 CG A:ASP245 3.4 4.0 1.0 CB A:ASP287 3.6 4.4 1.0 O A:HOH540 3.7 7.1 1.0 CE1 A:HIS220 3.9 4.6 1.0 CB A:ASP245 4.0 3.8 1.0 CG A:GLU217 4.1 3.2 1.0 O A:HOH739 4.1 9.0 1.0 CB A:GLU217 4.2 3.1 1.0 OE2 A:GLU217 4.3 3.6 1.0 OD1 A:ASP287 4.3 4.9 1.0 OD1 A:ASP245 4.3 3.7 1.0 C5 A:GLC403 4.4 6.9 1.0 CG A:GLU181 4.4 4.5 1.0 NE2 A:HIS220 4.5 4.7 1.0 C2 A:GLC403 4.5 7.0 1.0 MN A:MN402 4.5 6.5 1.0 ND1 A:HIS220 4.6 4.5 1.0

Manganese binding site 2 out of 2 in the A Form of Glucose Isomerase Collected at 100K.


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of A Form of Glucose Isomerase Collected at 100K. within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:6.5 occ:1.00 OE2 A:GLU217 2.1 3.6 1.0 OD1 A:ASP257 2.2 3.9 1.0 OD2 A:ASP255 2.2 5.0 1.0 O A:HOH540 2.3 7.1 1.0 OD1 A:ASP255 2.3 4.3 1.0 NE2 A:HIS220 2.4 4.7 1.0 CG A:ASP255 2.6 4.5 1.0 CD A:GLU217 2.8 3.5 1.0 OE1 A:GLU217 2.9 3.4 1.0 CD2 A:HIS220 3.0 4.1 1.0 CG A:ASP257 3.2 4.2 1.0 CE1 A:HIS220 3.4 4.6 1.0 OD2 A:ASP257 3.4 4.2 1.0 O A:HOH588 3.8 5.6 1.0 ND2 A:ASN247 4.0 3.0 1.0 O A:HOH613 4.0 4.4 1.0 O3 A:GLC403 4.0 6.2 1.0 CB A:ASP255 4.1 4.6 1.0 CG A:HIS220 4.2 4.0 1.0 OD2 A:ASP287 4.3 5.0 1.0 CG A:GLU217 4.3 3.2 1.0 ND1 A:HIS220 4.4 4.5 1.0 MN A:MN401 4.5 6.4 1.0 CB A:ASP257 4.6 4.1 1.0 O A:HOH525 4.7 16.3 1.0 CE A:LYS183 4.9 3.5 1.0 CA A:ASP255 5.0 4.4 1.0 CA A:ASP257 5.0 4.2 1.0

C.M.Lobley, J.Sandy, J.Sanchez-Weatherby, M.Mazzorana, T.Krojer, R.P.Nowak, T.L.Sorensen. A Generic Protocol For Protein Crystal Dehydration Using the HC1B Humidity Controller. Acta Crystallogr D Struct V. 72 629 2016BIOL.
ISSN: ISSN 2059-7983
PubMed: 27139626
DOI: 10.1107/S2059798316003065