Manganese in PDB 4wzq: Mutant K20E of Rna Dependent Rna Polymerase 3D From Foot-and-Mouth Disease Virus Complexed with Rna

The structure of Mutant K20E of Rna Dependent Rna Polymerase 3D From Foot-and-Mouth Disease Virus Complexed with Rna, PDB code: 4wzq was solved by N.Verdaguer, C.Ferrer-Orta, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	43.16 / 2.80
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	95.160, 95.160, 101.329, 90.00, 90.00, 120.00
R / Rfree (%)	24.9 / 27.1

The binding sites of Manganese atom in the Mutant K20E of Rna Dependent Rna Polymerase 3D From Foot-and-Mouth Disease Virus Complexed with Rna (pdb code 4wzq). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Mutant K20E of Rna Dependent Rna Polymerase 3D From Foot-and-Mouth Disease Virus Complexed with Rna, PDB code: 4wzq:

Manganese binding site 1 out of 1 in the Mutant K20E of Rna Dependent Rna Polymerase 3D From Foot-and-Mouth Disease Virus Complexed with Rna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Mutant K20E of Rna Dependent Rna Polymerase 3D From Foot-and-Mouth Disease Virus Complexed with Rna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:90.2 occ:1.00 OD1 A:ASP339 2.7 44.4 1.0 OD1 A:ASP238 3.1 52.0 1.0 OD1 A:ASP240 3.1 53.0 1.0 CG A:ASP339 3.6 43.6 1.0 O A:THR384 3.8 47.2 1.0 O A:VAL239 3.9 51.7 1.0 OD2 A:ASP339 4.1 43.5 1.0 O A:ILE340 4.2 46.8 1.0 CG A:ASP238 4.2 53.2 1.0 CG A:ASP240 4.2 53.9 1.0 N A:ILE340 4.3 44.8 1.0 CA A:ASP339 4.5 44.2 1.0 OD2 A:ASP238 4.6 53.9 1.0 O A:HOH605 4.6 51.7 1.0 CB A:ASP339 4.6 43.5 1.0 C A:THR384 4.7 47.0 1.0 OD2 A:ASP240 4.8 55.4 1.0 C A:VAL239 4.8 52.3 1.0 C A:ASP339 5.0 44.2 1.0

C.Ferrer-Orta, I.De La Higuera, F.Caridi, M.T.Sanchez-Aparicio, E.Moreno, C.Perales, K.Singh, S.G.Sarafianos, F.Sobrino, E.Domingo, N.Verdaguer. Multifunctionality of A Picornavirus Polymerase Domain: Nuclear Localization Signal and Nucleotide Recognition. J.Virol. 2015.
ISSN: ESSN 1098-5514
PubMed: 25903341
DOI: 10.1128/JVI.03283-14