Manganese in PDB 4wyl: Mutant K18E of 3D Polymerase From Foot-and-Moth Disease Virus

All present enzymatic activity of Mutant K18E of 3D Polymerase From Foot-and-Moth Disease Virus:
2.7.7.48;

The structure of Mutant K18E of 3D Polymerase From Foot-and-Moth Disease Virus, PDB code: 4wyl was solved by C.Ferrer-Orta, N.Verdaguer, I.De La Higuera, E.Domingo, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	45.81 / 2.00
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	91.627, 91.627, 117.852, 90.00, 90.00, 90.00
R / Rfree (%)	21.4 / 24.3

The binding sites of Manganese atom in the Mutant K18E of 3D Polymerase From Foot-and-Moth Disease Virus (pdb code 4wyl). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Mutant K18E of 3D Polymerase From Foot-and-Moth Disease Virus, PDB code: 4wyl:

Manganese binding site 1 out of 1 in the Mutant K18E of 3D Polymerase From Foot-and-Moth Disease Virus


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Mutant K18E of 3D Polymerase From Foot-and-Moth Disease Virus within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn505 b:66.3 occ:1.00 OD1 A:ASP240 2.2 29.4 1.0 OD1 A:ASP238 2.3 26.9 1.0 O A:VAL239 3.4 27.8 1.0 CG A:ASP238 3.4 26.6 1.0 CG A:ASP240 3.4 28.6 1.0 OD1 A:ASP339 3.6 21.4 1.0 OD2 A:ASP238 3.9 26.8 1.0 C A:VAL239 4.0 27.4 1.0 OD2 A:ASP240 4.0 29.6 1.0 O A:THR384 4.1 26.0 1.0 O A:ILE340 4.3 20.2 1.0 CB A:ALA367 4.3 38.4 1.0 OG1 A:THR384 4.4 28.6 1.0 N A:VAL239 4.4 27.0 1.0 C A:ASP238 4.5 26.5 1.0 N A:ASP240 4.5 27.2 1.0 CA A:ASP240 4.6 27.2 1.0 CB A:ASP238 4.6 26.3 1.0 CB A:ASP240 4.6 27.9 1.0 CG A:ASP339 4.7 21.2 1.0 CA A:ASP238 4.8 26.1 1.0 O A:ASP238 4.8 26.6 1.0 CA A:VAL239 4.9 27.3 1.0 N A:ILE340 4.9 20.6 1.0

C.Ferrer-Orta, I.De La Higuera, F.Caridi, M.T.Sanchez-Aparicio, E.Moreno, C.Perales, K.Singh, S.G.Sarafianos, F.Sobrino, E.Domingo, N.Verdaguer. Multifunctionality of A Picornavirus Polymerase Domain: Nuclear Localization Signal and Nucleotide Recognition. J.Virol. 2015.
ISSN: ESSN 1098-5514
PubMed: 25903341
DOI: 10.1128/JVI.03283-14