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Manganese in PDB 4pgx: Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese

Enzymatic activity of Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese

All present enzymatic activity of Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese:
2.7.7.7;

Protein crystallography data

The structure of Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese, PDB code: 4pgx was solved by M.C.Koag, S.Lee, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.97 / 2.08
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 50.969, 79.796, 55.635, 90.00, 107.37, 90.00
R / Rfree (%) 18.3 / 22.7

Other elements in 4pgx:

The structure of Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese also contains other interesting chemical elements:

Sodium (Na) 2 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese (pdb code 4pgx). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese, PDB code: 4pgx:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 4pgx

Go back to Manganese Binding Sites List in 4pgx
Manganese binding site 1 out of 2 in the Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn400

b:15.7
occ:1.00
OD1 A:ASP190 2.0 16.6 1.0
OD2 A:ASP192 2.1 15.1 1.0
O1A A:1FZ404 2.1 17.5 1.0
O2B A:1FZ404 2.1 14.2 1.0
O1G A:1FZ404 2.2 14.9 1.0
O A:HOH622 2.3 16.0 1.0
PB A:1FZ404 3.1 16.6 1.0
CG A:ASP192 3.1 14.5 1.0
CG A:ASP190 3.1 17.3 1.0
PA A:1FZ404 3.3 16.1 1.0
PG A:1FZ404 3.4 18.0 1.0
N3A A:1FZ404 3.5 15.2 1.0
OD1 A:ASP192 3.5 18.0 1.0
O3B A:1FZ404 3.6 15.1 1.0
OD2 A:ASP190 3.6 16.6 1.0
MN A:MN401 3.6 17.5 1.0
O A:HOH548 4.0 17.5 1.0
O3G A:1FZ404 4.1 17.2 1.0
O A:ASP190 4.1 19.2 1.0
C5' A:1FZ404 4.2 16.8 1.0
O A:HOH621 4.3 14.2 1.0
O5' A:1FZ404 4.3 16.6 1.0
OG A:SER180 4.3 21.5 1.0
N A:ASP190 4.3 20.8 1.0
C A:ASP190 4.3 19.6 1.0
N A:SER180 4.4 18.1 1.0
CB A:ASP192 4.4 16.9 1.0
CB A:ASP190 4.4 18.4 1.0
O1B A:1FZ404 4.4 17.0 1.0
CA A:GLY179 4.5 16.7 1.0
O2G A:1FZ404 4.5 17.1 1.0
O2A A:1FZ404 4.6 17.9 1.0
CA A:ASP190 4.6 16.3 1.0
O P:HOH102 4.8 16.1 1.0
N A:ASP192 4.8 17.1 1.0
N A:MET191 4.9 16.7 1.0

Manganese binding site 2 out of 2 in 4pgx

Go back to Manganese Binding Sites List in 4pgx
Manganese binding site 2 out of 2 in the Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Structure of Human Dna Polymerase Beta Complexed with G in the Template Base Paired with Incoming Non-Hydrolyzable Ttp and Manganese within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn401

b:17.5
occ:1.00
OD2 A:ASP256 2.0 19.2 1.0
OD1 A:ASP192 2.1 18.0 1.0
OD2 A:ASP190 2.1 16.6 1.0
O1A A:1FZ404 2.3 17.5 1.0
O P:HOH102 2.3 16.1 1.0
O3' P:DA10 2.4 20.4 1.0
CG A:ASP190 3.1 17.3 1.0
CG A:ASP256 3.1 21.4 1.0
CG A:ASP192 3.1 14.5 1.0
PA A:1FZ404 3.3 16.1 1.0
OD1 A:ASP190 3.3 16.6 1.0
OD2 A:ASP192 3.5 15.1 1.0
C3' P:DA10 3.6 19.9 1.0
MN A:MN400 3.6 15.7 1.0
O5' A:1FZ404 3.7 16.6 1.0
O2A A:1FZ404 3.8 17.9 1.0
CB A:ASP256 3.8 21.8 1.0
C5' A:1FZ404 4.1 16.8 1.0
OD1 A:ASP256 4.1 29.5 1.0
C4' P:DA10 4.2 20.9 1.0
C5' P:DA10 4.3 18.2 1.0
O A:HOH548 4.4 17.5 1.0
CB A:ASP192 4.5 16.9 1.0
CB A:ASP190 4.5 18.4 1.0
OP1 P:DA10 4.6 18.4 1.0
O5' P:DA10 4.6 17.6 1.0
O A:MET191 4.7 18.0 1.0
N3A A:1FZ404 4.7 15.2 1.0
C2' P:DA10 4.8 22.0 1.0
NH2 A:ARG254 4.8 31.4 1.0
O1G A:1FZ404 4.9 14.9 1.0
N A:ASP256 5.0 17.8 1.0

Reference:

M.C.Koag, K.Nam, S.Lee. The Spontaneous Replication Error and the Mismatch Discrimination Mechanisms of Human Dna Polymerase Beta. Nucleic Acids Res. V. 42 11233 2015.
ISSN: ESSN 1362-4962
PubMed: 25200079
DOI: 10.1093/NAR/GKU789
Page generated: Tue Dec 15 04:26:10 2020

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