Manganese in PDB 4nro: Crystal Structure of Human ALKBH5 in Complex with Alpha-Ketoglutarate

The structure of Crystal Structure of Human ALKBH5 in Complex with Alpha-Ketoglutarate, PDB code: 4nro was solved by C.Feng, Z.Chen, Y.Liu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 2.30
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	56.177, 56.177, 144.940, 90.00, 90.00, 90.00
R / Rfree (%)	23.7 / 25.6

The binding sites of Manganese atom in the Crystal Structure of Human ALKBH5 in Complex with Alpha-Ketoglutarate (pdb code 4nro). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Crystal Structure of Human ALKBH5 in Complex with Alpha-Ketoglutarate, PDB code: 4nro:

Manganese binding site 1 out of 1 in the Crystal Structure of Human ALKBH5 in Complex with Alpha-Ketoglutarate


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Human ALKBH5 in Complex with Alpha-Ketoglutarate within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn301 b:54.5 occ:1.00 OD1 A:ASP206 2.2 52.5 1.0 O1 A:AKG302 2.2 58.3 1.0 NE2 A:HIS266 2.2 51.9 1.0 NE2 A:HIS204 2.2 55.0 1.0 O5 A:AKG302 2.3 61.8 1.0 O A:HOH401 2.4 52.9 1.0 C2 A:AKG302 2.7 61.4 1.0 C1 A:AKG302 2.8 59.9 1.0 CE1 A:HIS266 3.0 49.5 1.0 CE1 A:HIS204 3.0 53.4 1.0 CG A:ASP206 3.2 51.6 1.0 CD2 A:HIS204 3.4 50.9 1.0 CD2 A:HIS266 3.4 48.0 1.0 OD2 A:ASP206 3.6 50.7 1.0 C3 A:AKG302 4.0 64.6 1.0 O2 A:AKG302 4.1 57.6 1.0 ND1 A:HIS266 4.2 48.6 1.0 ND1 A:HIS204 4.2 49.8 1.0 O A:HOH476 4.3 73.9 1.0 O A:HOH445 4.4 63.1 1.0 CG A:HIS204 4.4 50.6 1.0 CG A:HIS266 4.4 47.1 1.0 C4 A:AKG302 4.5 61.5 1.0 CB A:ASP206 4.6 50.3 1.0 N A:ASP206 4.9 46.2 1.0 CA A:ASP206 4.9 48.1 1.0

C.Feng, Y.Liu, G.Wang, Z.Deng, Q.Zhang, W.Wu, Y.Tong, C.Cheng, Z.Chen. Crystal Structures of Human Rna Demethylase ALKBH5 Reveal Basis For Substrate Recognition J.Biol.Chem. 2014.
ISSN: ESSN 1083-351X
DOI: 10.1074/JBC.M113.546168