Manganese in PDB 4n56: Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna

Enzymatic activity of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna

All present enzymatic activity of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna:
2.7.7.7;

Protein crystallography data

The structure of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna, PDB code: 4n56 was solved by E.Y.Wu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

*Resolution Low / High (Å)*	47.65 / 2.20
*Space group*	P 3₁ 2 1
*Cell size a, b, c (Å), α, β, γ (°)*	110.040, 110.040, 91.290, 90.00, 90.00, 120.00
*R / R_free (%)*	22.3 / 27.3

Other elements in 4n56:

The structure of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna also contains other interesting chemical elements:

Magnesium

(Mg)

1 atom

Manganese Binding Sites:

The binding sites of Manganese atom in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna (pdb code 4n56). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna, PDB code: 4n56:

Manganese binding site 1 out of 1 in 4n56

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Manganese Binding Sites List in 4n56

Manganese binding site 1 out of 1 in the Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna

Mono view

Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Binary Complex Structure of Klenow Fragment of Taq Dna Polymerase I707L Mutant (CS3C Klentaq) with Dna within 5.0Å range:

probe	atom	residue	distance (Å)	B	Occ
C:Mn301 b:58.8 occ:1.00	OP2	C:DG204	1.9	75.2	1.0
	N7	C:DG205	2.6	40.3	1.0
	P	C:DG204	3.1	65.8	1.0
	O5'	C:DG204	3.1	81.5	1.0
	C8	C:DG205	3.4	46.2	1.0
	C5	C:DG205	3.7	41.0	1.0
	O3'	C:DA203	3.9	78.4	1.0
	O6	C:DG205	4.0	37.4	1.0
	N7	C:DG206	4.1	42.4	1.0
	C6	C:DG205	4.2	41.6	1.0
	OP1	C:DG204	4.3	69.3	1.0
	C5'	C:DG204	4.4	79.3	1.0
	O6	C:DG206	4.5	40.2	1.0
	OP2	C:DG205	4.5	50.0	1.0
	N9	C:DG205	4.6	48.0	1.0
	C4	C:DG205	4.8	40.1	1.0
	C3'	C:DA203	4.8	71.7	1.0
	C4'	C:DG204	4.9	80.5	1.0
	C2'	C:DA203	4.9	75.1	1.0
	C5	C:DG206	4.9	42.7	1.0

Reference:

E.Y.Wu, A.R.Walsh, E.C.Materne, E.P.Hiltner, B.Zielinski, B.R.Miller, L.Mawby, E.Modeste, C.A.Parish, W.M.Barnes, M.B.Kermekchiev. A Conservative Isoleucine to Leucine Mutation Causes Major Rearrangements and Cold Sensitivity in KLENTAQ1 Dna Polymerase. Biochemistry V. 54 881 2015.
ISSN: ISSN 0006-2960
PubMed: 25537790
DOI: 10.1021/BI501198F

Page generated: Sat Oct 5 20:29:27 2024

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