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Manganese in PDB 4mu4: The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution

Enzymatic activity of The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution

All present enzymatic activity of The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution:
4.2.1.19;

Protein crystallography data

The structure of The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution, PDB code: 4mu4 was solved by C.Bisson, K.L.Britton, S.E.Sedelnikova, P.J.Baker, D.W.Rice, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 65.18 / 1.41
Space group P 4 3 2
Cell size a, b, c (Å), α, β, γ (°) 112.900, 112.900, 112.900, 90.00, 90.00, 90.00
R / Rfree (%) 12.3 / 15.4

Manganese Binding Sites:

The binding sites of Manganese atom in the The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution (pdb code 4mu4). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution, PDB code: 4mu4:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 4mu4

Go back to Manganese Binding Sites List in 4mu4
Manganese binding site 1 out of 2 in the The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn301

b:8.8
occ:1.00
N2 A:IYP303 2.3 11.9 1.0
NE2 A:HIS74 2.3 8.9 1.0
O2 A:IYP303 2.3 14.2 1.0
C6 A:IYP303 3.1 11.2 1.0
CE1 A:HIS74 3.2 10.2 1.0
C4 A:IYP303 3.3 16.4 1.0
CD2 A:HIS74 3.4 8.6 1.0
C2 A:IYP303 3.4 17.2 1.0
C3 A:IYP303 3.9 31.7 1.0
N1 A:IYP303 4.3 9.8 1.0
ND1 A:HIS74 4.4 9.7 1.0
C5 A:IYP303 4.4 12.5 1.0
CG A:HIS74 4.5 8.5 1.0
C1 A:IYP303 4.6 18.1 1.0
NE2 A:GLN21 4.9 24.0 0.5

Manganese binding site 2 out of 2 in 4mu4

Go back to Manganese Binding Sites List in 4mu4
Manganese binding site 2 out of 2 in the The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of The Form B Structure of An E21Q Catalytic Mutant of A. Thaliana IGPD2 in Complex with MN2+ and Its Substrate, 2R3S-Igp, to 1.41 A Resolution within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn302

b:8.3
occ:1.00
N1 A:IYP303 2.2 9.8 1.0
O A:HOH401 2.2 10.7 1.0
NE2 A:HIS73 2.2 8.3 1.0
NE2 A:HIS145 2.3 9.0 1.0
OE1 A:GLU77 2.4 9.6 1.0
C5 A:IYP303 3.0 12.5 1.0
CE1 A:HIS73 3.2 7.9 1.0
CD2 A:HIS73 3.2 9.8 1.0
CD2 A:HIS145 3.3 8.8 1.0
C6 A:IYP303 3.3 11.2 1.0
CE1 A:HIS145 3.3 10.1 1.0
CD A:GLU77 3.3 9.8 1.0
OE2 A:GLU77 3.7 9.6 1.0
C4 A:IYP303 4.2 16.4 1.0
ND1 A:HIS73 4.3 8.1 1.0
ND1 A:HIS145 4.4 10.7 1.0
N2 A:IYP303 4.4 11.9 1.0
CG A:HIS73 4.4 7.0 1.0
CG A:HIS145 4.4 9.4 1.0
CG A:GLU77 4.6 7.3 1.0
CB A:GLU77 4.8 8.6 1.0
O A:HOH405 4.9 9.1 1.0

Reference:

C.Bisson, K.L.Britton, S.E.Sedelnikova, H.F.Rodgers, T.C.Eadsforth, R.Viner, T.R.Hawkes, P.J.Baker, D.W.Rice. A New Role For Metal Ions in Enzyme Catalysis To Be Published.
Page generated: Tue Dec 15 04:24:47 2020

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