Manganese in PDB 4krv: Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac

All present enzymatic activity of Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac:
2.4.1.22;

The structure of Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac, PDB code: 4krv was solved by B.Ramakrishnan, P.K.Qasba, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.64 / 2.40
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	49.282, 88.903, 139.734, 90.00, 90.00, 90.00
R / Rfree (%)	18.8 / 24.9

The binding sites of Manganese atom in the Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac (pdb code 4krv). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac, PDB code: 4krv:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn501 b:17.7 occ:1.00 O1A A:UDH502 2.1 13.8 1.0 OD2 A:ASP254 2.2 16.4 1.0 O A:HOH664 2.2 14.7 1.0 O1B A:UDH502 2.2 14.9 1.0 NE2 A:HIS347 2.3 19.6 1.0 NE2 A:HIS344 2.3 20.3 1.0 CG A:ASP254 3.0 15.5 1.0 CE1 A:HIS344 3.1 19.3 1.0 CE1 A:HIS347 3.2 15.3 1.0 CD2 A:HIS347 3.3 15.9 1.0 PB A:UDH502 3.3 23.8 1.0 OD1 A:ASP254 3.3 14.3 1.0 PA A:UDH502 3.4 16.4 1.0 CD2 A:HIS344 3.4 19.9 1.0 O3A A:UDH502 3.6 20.4 1.0 O2B A:UDH502 3.9 27.4 1.0 O3' A:UDH502 4.0 16.6 1.0 O2A A:UDH502 4.2 17.2 1.0 O A:HOH669 4.2 19.6 1.0 CB A:ASP254 4.3 14.0 1.0 ND1 A:HIS344 4.3 23.5 1.0 ND1 A:HIS347 4.3 20.7 1.0 CG A:HIS347 4.4 18.8 1.0 NZ A:LYS279 4.4 26.0 1.0 CG A:HIS344 4.5 21.9 1.0 O5' A:UDH502 4.5 19.7 1.0 NH2 A:ARG191 4.6 13.5 1.0 NH1 A:ARG191 4.6 18.2 1.0 C3B A:UDH502 4.6 15.8 1.0 O3B A:UDH502 4.6 28.7 1.0 C5B A:UDH502 4.7 13.6 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Catalytic Domain of Bovine BETA1,4- Galactosyltransferase Mutant M344H-GALT1 Complex with 6-Sulfo-Glcnac within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn501 b:20.6 occ:1.00 O1B B:UDH502 2.1 21.5 1.0 O1A B:UDH502 2.1 16.1 1.0 OD2 B:ASP254 2.1 20.9 1.0 NE2 B:HIS347 2.2 23.4 1.0 NE2 B:HIS344 2.2 20.9 1.0 O B:HOH671 2.7 26.0 1.0 CG B:ASP254 2.9 21.5 1.0 CD2 B:HIS347 3.1 24.6 1.0 OD1 B:ASP254 3.2 18.1 1.0 CD2 B:HIS344 3.2 21.2 1.0 CE1 B:HIS344 3.2 22.2 1.0 CE1 B:HIS347 3.3 19.6 1.0 PB B:UDH502 3.4 30.1 1.0 PA B:UDH502 3.4 20.4 1.0 O3A B:UDH502 3.7 34.1 1.0 O3' B:UDH502 4.0 18.9 1.0 CB B:ASP254 4.1 18.5 1.0 NZ B:LYS279 4.2 22.4 1.0 O2A B:UDH502 4.3 22.3 1.0 CG B:HIS347 4.3 23.9 1.0 O2B B:UDH502 4.3 33.2 1.0 ND1 B:HIS344 4.3 21.6 1.0 ND1 B:HIS347 4.4 22.0 1.0 CG B:HIS344 4.4 20.2 1.0 NH2 B:ARG191 4.4 17.2 1.0 NH1 B:ARG191 4.4 23.6 1.0 O3B B:UDH502 4.4 30.7 1.0 O5' B:UDH502 4.6 22.9 1.0 C3B B:UDH502 4.6 17.7 1.0 C5B B:UDH502 4.7 20.2 1.0 CD B:LYS279 4.8 26.7 1.0 O B:ILE345 4.9 23.1 1.0 CZ B:ARG191 4.9 19.9 1.0

B.Ramakrishnan, A.J.Moncrief, T.A.Davis, L.A.Holland, P.K.Qasba. Investigations on Beta 1,4-Galactosyltransferase I Using 6-Sulfo-Glcnac As An Acceptor Sugar Substrate. Glycoconj J V. 30 835 2013.
PubMed: 23942731
DOI: 10.1007/S10719-013-9488-4