Manganese in PDB 4klu: Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H

All present enzymatic activity of Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H:
2.7.7.7;

The structure of Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H, PDB code: 4klu was solved by B.D.Freudenthal, W.A.Beard, D.D.Shock, S.H.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.99 / 1.97
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	55.222, 80.105, 55.812, 90.00, 109.64, 90.00
R / Rfree (%)	21.2 / 25.1

The structure of Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H also contains other interesting chemical elements:

Sodium

(Na)

2 atoms

The binding sites of Manganese atom in the Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H (pdb code 4klu). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H, PDB code: 4klu:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H within 5.0Å range: probe atom residue distance (Å) B Occ T:Mn101 b:38.7 occ:1.00 O T:HOH241 2.3 37.8 1.0 O T:HOH239 2.3 34.7 1.0 N7 T:DG3 2.4 26.7 1.0 O T:HOH221 2.4 32.9 1.0 O T:HOH229 2.4 39.7 1.0 O T:HOH224 2.5 39.2 1.0 C8 T:DG3 3.2 25.6 1.0 C5 T:DG3 3.5 26.6 1.0 O6 T:DG3 3.9 24.9 1.0 C6 T:DG3 4.1 25.6 1.0 OP2 T:DG3 4.1 42.3 1.0 O T:HOH230 4.2 34.8 1.0 O D:HOH211 4.4 27.8 1.0 N9 T:DG3 4.5 27.3 1.0 N6 T:DA4 4.5 18.4 1.0 C4 T:DG3 4.6 22.5 1.0 C2' T:DC2 4.8 35.2 1.0 C6 T:DC2 4.8 29.5 1.0

Manganese binding site 2 out of 2 in the Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Dna Polymerase Beta Mismatched Product Complex with MN2+, 15 H within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn101 b:28.0 occ:1.00 O D:HOH218 2.0 26.1 1.0 O D:HOH202 2.0 23.4 1.0 O D:HOH203 2.1 27.5 1.0 O D:HOH201 2.3 26.4 1.0 N7 D:DG4 2.4 23.6 1.0 O D:HOH208 2.4 25.6 1.0 C8 D:DG4 3.2 22.4 1.0 C5 D:DG4 3.5 24.2 1.0 O6 D:DG4 3.7 26.9 1.0 C6 D:DG4 4.0 24.7 1.0 O6 D:DG5 4.3 25.3 1.0 N9 D:DG4 4.5 24.8 1.0 OP2 D:DG4 4.6 27.2 1.0 C4 D:DG4 4.6 23.8 1.0 C2' D:DC3 4.8 26.2 1.0 C6 D:DC3 4.8 18.6 1.0

B.D.Freudenthal, W.A.Beard, D.D.Shock, S.H.Wilson. Observing A Dna Polymerase Choose Right From Wrong. Cell(Cambridge,Mass.) V. 154 157 2013.
ISSN: ISSN 0092-8674
PubMed: 23827680
DOI: 10.1016/J.CELL.2013.05.048