Manganese in PDB 4g24: Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn

All present enzymatic activity of Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn:
3.1.26.5;

The structure of Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn, PDB code: 4g24 was solved by M.Koutmos, M.J.Howard, C.A.Fierke, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	36.19 / 1.95
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	41.750, 112.475, 138.762, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 22.7

The structure of Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn also contains other interesting chemical elements:

Zinc

(Zn)

1 atom

The binding sites of Manganese atom in the Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn (pdb code 4g24). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn, PDB code: 4g24:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1002 b:38.9 occ:1.00 O A:HOH1414 2.1 26.4 1.0 O A:HOH1218 2.1 39.2 1.0 OD1 A:ASP475 2.3 35.1 1.0 O A:HOH1423 2.3 32.1 1.0 O2 A:ACA1004 2.3 37.7 0.5 O2 A:ACA1004 2.4 42.3 0.5 O1 A:ACA1004 2.6 44.3 0.5 C1 A:ACA1004 2.9 42.7 0.5 CG A:ASP475 3.3 35.7 1.0 MN A:MN1003 3.5 53.2 0.8 C1 A:ACA1004 3.5 39.5 0.5 OD2 A:ASP475 3.6 37.8 1.0 O A:HOH1271 3.8 47.7 1.0 OD2 A:ASP474 3.9 40.1 1.0 OD2 A:ASP493 4.0 34.0 1.0 O1 A:ACA1004 4.1 35.7 0.5 NE1 A:TRP478 4.1 28.0 1.0 OD2 A:ASP399 4.2 25.4 1.0 OD1 A:ASP474 4.2 45.3 1.0 OD1 A:ASP399 4.2 26.7 1.0 C2 A:ACA1004 4.4 45.3 0.5 CG A:ASP474 4.4 43.5 1.0 CZ2 A:TRP478 4.4 27.8 1.0 CG A:ASP399 4.6 23.7 1.0 CE2 A:TRP478 4.7 27.1 1.0 CB A:ASP475 4.7 34.1 1.0 C2 A:ACA1004 4.7 38.3 0.5 N A:ASP475 4.8 31.9 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Proteinaceous Rnase P 1 (PRORP1) From A. Thaliana with Mn within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1003 b:53.2 occ:0.80 OD2 A:ASP493 2.3 34.0 1.0 OD2 A:ASP475 2.6 37.8 1.0 O1 A:ACA1004 3.0 44.3 0.5 CG A:ASP493 3.4 35.8 1.0 CG A:ASP475 3.5 35.7 1.0 MN A:MN1002 3.5 38.9 1.0 O A:HOH1423 3.5 32.1 1.0 OD1 A:ASP475 3.7 35.1 1.0 OD2 A:ASP497 3.7 20.0 1.0 OD1 A:ASP493 3.8 36.9 1.0 C1 A:ACA1004 4.1 42.7 0.5 C5 A:ACA1004 4.1 49.3 0.5 CE2 A:PHE500 4.1 45.7 1.0 O A:HOH1271 4.2 47.7 1.0 O A:HOH1429 4.4 46.7 1.0 O2 A:ACA1004 4.4 37.7 0.5 C4 A:ACA1004 4.4 50.7 0.5 C6 A:ACA1004 4.6 47.1 0.5 O A:HOH1218 4.6 39.2 1.0 CZ A:PHE500 4.6 43.9 1.0 CG A:ASP497 4.7 20.0 1.0 CB A:ASP493 4.7 33.0 1.0 O2 A:ACA1004 4.7 42.3 0.5 O1 A:ACA1004 4.7 35.7 0.5 C1 A:ACA1004 4.8 39.5 0.5 CB A:ASP475 4.8 34.1 1.0

M.J.Howard, W.H.Lim, C.A.Fierke, M.Koutmos. Mitochondrial Ribonuclease P Structure Provides Insight Into the Evolution of Catalytic Strategies For Precursor-Trna 5' Processing. Proc.Natl.Acad.Sci.Usa V. 109 16149 2012.
ISSN: ISSN 0027-8424
PubMed: 22991464
DOI: 10.1073/PNAS.1209062109