Manganese in PDB 4ff2: N4 Mini-Vrnap Transcription Initiation Complex, 2 Min After Soaking Gtp, Atp and Mn

The structure of N4 Mini-Vrnap Transcription Initiation Complex, 2 Min After Soaking Gtp, Atp and Mn, PDB code: 4ff2 was solved by K.S.Murakami, R.S.Basu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	47.67 / 2.00
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	81.908, 111.437, 275.760, 90.00, 90.00, 90.00
R / Rfree (%)	20.8 / 25.5

The binding sites of Manganese atom in the N4 Mini-Vrnap Transcription Initiation Complex, 2 Min After Soaking Gtp, Atp and Mn (pdb code 4ff2). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the N4 Mini-Vrnap Transcription Initiation Complex, 2 Min After Soaking Gtp, Atp and Mn, PDB code: 4ff2:

Manganese binding site 1 out of 1 in the N4 Mini-Vrnap Transcription Initiation Complex, 2 Min After Soaking Gtp, Atp and Mn


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of N4 Mini-Vrnap Transcription Initiation Complex, 2 Min After Soaking Gtp, Atp and Mn within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1202 b:55.3 occ:1.00 OD2 A:ASP951 2.1 39.5 1.0 O3G A:ATP1201 2.3 57.1 1.0 OD2 A:ASP559 2.3 31.8 0.5 O A:GLY560 2.3 15.3 1.0 O1B A:ATP1201 2.4 47.0 1.0 O1A A:ATP1201 2.4 50.8 1.0 OD2 A:ASP559 3.2 33.7 0.5 CG A:ASP951 3.2 32.2 1.0 CG A:ASP559 3.3 33.0 0.5 C A:GLY560 3.3 19.5 1.0 CG A:ASP559 3.4 32.1 0.5 OD1 A:ASP559 3.5 34.2 0.5 PB A:ATP1201 3.6 55.0 1.0 OD1 A:ASP951 3.6 29.9 1.0 N A:GLY560 3.7 17.7 1.0 PA A:ATP1201 3.7 55.5 1.0 PG A:ATP1201 3.7 55.5 1.0 CA A:GLY560 3.8 16.7 1.0 O3A A:ATP1201 3.8 1.0 1.0 OD1 A:ASP559 3.9 34.4 0.5 O3B A:ATP1201 4.1 0.7 1.0 C A:ASP559 4.2 20.1 0.5 C5' A:ATP1201 4.3 41.4 1.0 C A:ASP559 4.3 20.2 0.5 CB A:ASP559 4.4 27.4 0.5 CB A:ASP559 4.5 26.2 0.5 CB A:ASN563 4.5 11.0 1.0 CB A:ASP951 4.5 25.4 1.0 O5' A:ATP1201 4.5 49.8 1.0 N A:VAL561 4.5 15.2 1.0 O A:ASP951 4.5 15.8 1.0 O1G A:ATP1201 4.5 52.3 1.0 N A:THR562 4.7 11.5 1.0 O2G A:ATP1201 4.7 62.6 1.0 N A:ASN563 4.8 9.9 1.0 CA A:ASP559 4.8 22.3 0.5 CA A:ASP559 4.8 22.3 0.5 O A:ASP559 4.8 19.0 0.5 O3' C:5GP101 4.9 56.9 1.0 O2B A:ATP1201 4.9 53.9 1.0 O2A A:ATP1201 4.9 57.8 1.0 O A:ASP559 4.9 18.8 0.5 CA A:VAL561 4.9 14.6 1.0

R.S.Basu, K.S.Murakami. Watching the Bacteriophage N4 Rna Polymerase Transcription By Time-Dependent Soak-Trigger-Freeze X-Ray Crystallography. J.Biol.Chem. V. 288 3305 2013.
ISSN: ISSN 0021-9258
PubMed: 23235152
DOI: 10.1074/JBC.M112.387712