Manganese in PDB 4f2d: Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol

All present enzymatic activity of Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol:
5.3.1.4;

The structure of Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol, PDB code: 4f2d was solved by B.A.Manjasetty, S.K.Burley, S.C.Almo, M.R.Chance, New York Sgx Researchcenter For Structural Genomics (Nysgxrc), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 2.30
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	116.472, 116.472, 214.810, 90.00, 90.00, 120.00
R / Rfree (%)	21.2 / 25.5

The binding sites of Manganese atom in the Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol (pdb code 4f2d). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol, PDB code: 4f2d:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in the Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn601 b:45.7 occ:1.00 O5 A:RB0602 2.0 43.5 1.0 NE2 A:HIS450 2.1 24.3 1.0 OE1 A:GLU306 2.2 32.2 1.0 NE2 A:HIS350 2.2 20.9 1.0 OE1 A:GLU333 2.3 31.9 1.0 CD2 A:HIS350 3.0 21.9 1.0 CD2 A:HIS450 3.0 23.6 1.0 O4 A:RB0602 3.1 50.8 1.0 CD A:GLU306 3.1 28.1 1.0 C5 A:RB0602 3.2 47.7 1.0 CE1 A:HIS450 3.2 24.3 1.0 CD A:GLU333 3.2 27.5 1.0 CE1 A:HIS350 3.3 22.4 1.0 C4 A:RB0602 3.4 46.8 1.0 OE2 A:GLU306 3.5 29.9 1.0 OE2 A:GLU333 3.7 32.4 1.0 CE1 A:HIS449 4.0 25.5 1.0 ND1 A:HIS449 4.2 28.0 1.0 CG A:HIS350 4.2 22.5 1.0 CG A:GLU333 4.2 25.6 1.0 CG A:HIS450 4.2 23.2 1.0 ND1 A:HIS450 4.3 21.4 1.0 O A:HOH788 4.3 37.4 1.0 ND1 A:HIS350 4.3 22.7 1.0 CG A:GLU306 4.4 26.8 1.0 CZ A:PHE331 4.5 17.3 1.0 O A:HOH765 4.7 25.6 0.5 CB A:GLU333 4.7 23.3 1.0 C3 A:RB0602 4.9 49.2 1.0

Manganese binding site 2 out of 3 in the Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn601 b:52.6 occ:1.00 NE2 B:HIS350 2.0 30.8 1.0 OE2 B:GLU306 2.2 31.2 1.0 O5 B:RB0602 2.2 43.0 1.0 NE2 B:HIS450 2.3 27.3 1.0 OE1 B:GLU333 2.6 32.7 1.0 CD2 B:HIS350 2.9 29.0 1.0 CD B:GLU306 3.0 31.5 1.0 CE1 B:HIS350 3.0 32.1 1.0 OE1 B:GLU306 3.2 34.0 1.0 CD2 B:HIS450 3.2 25.1 1.0 CE1 B:HIS450 3.2 27.6 1.0 O4 B:RB0602 3.3 65.4 1.0 C5 B:RB0602 3.4 48.2 1.0 CD B:GLU333 3.5 29.6 1.0 C4 B:RB0602 3.5 52.2 1.0 OE2 B:GLU333 4.0 31.8 1.0 CG B:HIS350 4.1 28.3 1.0 ND1 B:HIS350 4.1 30.9 1.0 CE1 B:HIS449 4.2 23.1 1.0 CG B:GLU306 4.2 29.7 1.0 ND1 B:HIS450 4.4 27.2 1.0 CG B:HIS450 4.4 25.3 1.0 ND1 B:HIS449 4.4 23.3 1.0 CZ B:PHE331 4.4 20.1 1.0 CG B:GLU333 4.5 27.3 1.0 O B:HOH787 4.9 49.7 1.0 CE2 B:PHE331 4.9 20.7 1.0 CB B:GLU333 4.9 25.8 1.0

Manganese binding site 3 out of 3 in the Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn601 b:80.6 occ:0.70 OE1 C:GLU306 2.1 34.6 1.0 NE2 C:HIS350 2.1 61.4 1.0 NE2 C:HIS450 2.5 50.3 1.0 OE2 C:GLU333 2.5 58.1 1.0 O5 C:RB0602 2.6 56.2 0.5 O4 C:RB0602 3.0 59.3 0.5 CD C:GLU306 3.0 32.2 1.0 CD2 C:HIS350 3.0 61.5 1.0 CE1 C:HIS350 3.1 59.2 1.0 C4 C:RB0602 3.2 52.4 0.5 OE2 C:GLU306 3.3 37.6 1.0 C5 C:RB0602 3.3 54.8 0.5 CE1 C:HIS450 3.4 37.6 1.0 CD2 C:HIS450 3.4 51.7 1.0 CD C:GLU333 3.5 56.0 1.0 OE1 C:GLU333 4.0 59.2 1.0 CG C:HIS350 4.2 60.2 1.0 ND1 C:HIS350 4.2 60.2 1.0 CG C:GLU306 4.3 30.5 0.5 CD2 C:HIS449 4.4 58.0 1.0 NE2 C:HIS449 4.4 57.5 1.0 ND1 C:HIS450 4.6 52.4 1.0 CG C:GLU333 4.6 53.9 1.0 CG C:HIS450 4.6 54.8 1.0 C3 C:RB0602 4.7 54.7 0.5 CZ C:PHE331 4.8 48.9 1.0

B.A.Manjasetty, S.K.Burley, S.C.Almo, M.R.Chance. Crystal Structure of Escherichia Coli L-Arabinose Isomerase (Ecai) Complexed with Ribitol To Be Published.