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Manganese in PDB 4dqr: Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+
Enzymatic activity of Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+
All present enzymatic activity of Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+:
2.7.7.7;
2.7.7.7;
Protein crystallography data
The structure of Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+, PDB code: 4dqr
was solved by
W.Wang,
L.S.Beese,
with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:
| Resolution Low / High (Å) | 36.79 / 1.95 |
| Space group | P 21 21 21 |
| Cell size a, b, c (Å), α, β, γ (°) | 93.897, 108.846, 149.771, 90.00, 90.00, 90.00 |
| R / Rfree (%) | 16.6 / 19.8 |
Manganese Binding Sites:
The binding sites of Manganese atom in the Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+
(pdb code 4dqr). This binding sites where shown within
5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+, PDB code: 4dqr:
Jump to Manganese binding site number: 1; 2;
In total 2 binding sites of Manganese where determined in the Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+, PDB code: 4dqr:
Jump to Manganese binding site number: 1; 2;
Manganese binding site 1 out of 2 in 4dqr
Go back to
Manganese Binding Sites List in 4dqr
Manganese binding site 1 out
of 2 in the Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Manganese with other atoms in the Mn binding
site number 1 of Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+ within 5.0Å range:
|
Manganese binding site 2 out of 2 in 4dqr
Go back to
Manganese Binding Sites List in 4dqr
Manganese binding site 2 out
of 2 in the Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+
Mono view
Stereo pair view
Mono view
Stereo pair view
A full contact list of Manganese with other atoms in the Mn binding
site number 2 of Ternary Complex of Bacillus Dna Polymerase I Large Fragment E658A, Dna Duplex, and Rctp (Paired with Dg of Template) in Presence of MN2+ within 5.0Å range:
|
Reference:
W.Wang,
E.Y.Wu,
H.W.Hellinga,
L.S.Beese.
Structural Factors That Determine Selectivity of A High Fidelity Dna Polymerase For Deoxy-, Dideoxy-, and Ribonucleotides. J.Biol.Chem. V. 287 28215 2012.
ISSN: ISSN 0021-9258
PubMed: 22648417
DOI: 10.1074/JBC.M112.366609
Page generated: Tue Dec 15 04:19:23 2020
ISSN: ISSN 0021-9258
PubMed: 22648417
DOI: 10.1074/JBC.M112.366609
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