Manganese in PDB 4c20: L-Fucose Isomerase

All present enzymatic activity of L-Fucose Isomerase:
5.3.1.25;

The structure of L-Fucose Isomerase, PDB code: 4c20 was solved by M.A.Higgins, M.D.L.Suits, C.Marsters, A.B.Boraston, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	39.07 / 2.41
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	151.931, 151.931, 408.853, 90.00, 90.00, 120.00
R / Rfree (%)	15.415 / 19.045

The binding sites of Manganese atom in the L-Fucose Isomerase (pdb code 4c20). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the L-Fucose Isomerase, PDB code: 4c20:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the L-Fucose Isomerase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of L-Fucose Isomerase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn601 b:41.7 occ:1.00 O A:HOH2259 2.1 28.7 1.0 OD1 A:ASP359 2.2 32.7 1.0 NE2 A:HIS525 2.2 23.6 1.0 O1 A:EDO1594 2.3 60.0 1.0 OD2 A:ASP359 2.7 39.0 1.0 CG A:ASP359 2.8 31.6 1.0 OE1 A:GLU335 2.9 32.1 1.0 C1 A:EDO1594 2.9 58.8 1.0 CE1 A:HIS525 3.2 22.6 1.0 CD2 A:HIS525 3.2 23.9 1.0 C2 A:EDO1594 3.6 59.1 1.0 CD A:GLU335 3.7 32.0 1.0 OE2 A:GLU335 3.7 38.6 1.0 O2 A:EDO1594 3.8 61.8 1.0 ND2 A:ASN524 3.9 24.7 1.0 OG A:SER391 4.0 34.2 1.0 ND1 A:HIS525 4.3 23.5 1.0 CB A:ASP359 4.3 29.0 1.0 OD1 A:ASN390 4.3 22.6 1.0 CG A:HIS525 4.3 23.8 1.0 CB A:SER391 4.7 29.4 1.0 CE1 A:PHE357 4.9 22.3 1.0 CA A:ASP359 5.0 26.3 1.0

Manganese binding site 2 out of 2 in the L-Fucose Isomerase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of L-Fucose Isomerase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn601 b:47.4 occ:1.00 O2 B:EDO1593 1.7 45.1 1.0 O B:HOH2250 2.1 35.9 1.0 OD1 B:ASP359 2.2 40.1 1.0 NE2 B:HIS525 2.4 26.4 1.0 OE1 B:GLU335 2.8 32.5 1.0 O1 B:EDO1593 2.8 47.0 1.0 OD2 B:ASP359 2.9 49.6 1.0 CG B:ASP359 2.9 37.2 1.0 C2 B:EDO1593 2.9 47.3 1.0 OE2 B:GLU335 3.3 29.4 1.0 CD2 B:HIS525 3.3 24.4 1.0 CE1 B:HIS525 3.3 24.7 1.0 CD B:GLU335 3.4 28.5 1.0 C1 B:EDO1593 3.5 45.6 1.0 OG B:SER391 3.9 31.2 1.0 ND2 B:ASN524 3.9 27.1 1.0 OD1 B:ASN390 4.1 32.2 1.0 CB B:ASP359 4.4 32.4 1.0 ND1 B:HIS525 4.5 24.7 1.0 CG B:HIS525 4.5 24.4 1.0 CB B:SER391 4.5 30.9 1.0 N B:SER391 4.8 29.6 1.0 CE1 B:PHE357 4.8 22.6 1.0 CG B:GLU335 4.9 25.5 1.0 CA B:ASP359 5.0 27.9 1.0

M.A.Higgins, M.D.Suits, C.Marsters, A.B.Boraston. Structural and Functional Analysis of Fucose-Processing Enzymes From Streptococcus Pneumoniae. J.Mol.Biol. V. 426 1469 2014.
ISSN: ISSN 0022-2836
PubMed: 24333485
DOI: 10.1016/J.JMB.2013.12.006