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Manganese in PDB 4c1l: Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant

Enzymatic activity of Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant

All present enzymatic activity of Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant:
2.5.1.54;

Protein crystallography data

The structure of Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant, PDB code: 4c1l was solved by A.R.Nazmi, L.R.Schofield, R.C.J.Dobson, G.B.Jameson, E.J.Parker, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 43.97 / 1.80
Space group I 2 2 2
Cell size a, b, c (Å), α, β, γ (°) 87.942, 110.923, 143.531, 90.00, 90.00, 90.00
R / Rfree (%) 16.419 / 18.331

Other elements in 4c1l:

The structure of Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant also contains other interesting chemical elements:

Potassium (K) 2 atoms
Chlorine (Cl) 4 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant (pdb code 4c1l). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant, PDB code: 4c1l:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 4c1l

Go back to Manganese Binding Sites List in 4c1l
Manganese binding site 1 out of 2 in the Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn1263

b:27.1
occ:0.50
OE2 A:GLU227 2.0 27.5 1.0
OD2 A:ASP238 2.3 43.8 1.0
SG A:CYS31 2.4 26.3 1.0
NE2 A:HIS201 2.6 27.9 1.0
CD A:GLU227 3.0 23.7 1.0
CE1 A:HIS201 3.2 27.7 1.0
CB A:CYS31 3.4 22.8 1.0
CG A:ASP238 3.4 41.0 1.0
OE1 A:GLU227 3.4 25.7 1.0
CD2 A:HIS201 3.7 26.9 1.0
CB A:ASP238 4.0 39.3 1.0
CA A:CYS31 4.1 20.5 1.0
NE2 A:GLN241 4.2 33.9 1.0
NZ A:LYS60 4.2 24.4 0.6
OD1 A:ASP238 4.3 41.0 1.0
CG A:GLU227 4.4 21.3 1.0
ND1 A:HIS201 4.4 26.1 1.0
CG A:HIS201 4.7 24.4 1.0
NH2 A:ARG55 4.7 24.4 0.5
NH2 A:ARG55 4.9 27.5 0.5
C A:CYS31 4.9 20.4 1.0
CE A:LYS60 4.9 24.3 0.6

Manganese binding site 2 out of 2 in 4c1l

Go back to Manganese Binding Sites List in 4c1l
Manganese binding site 2 out of 2 in the Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Pyrococcus Furiosus 3-Deoxy-D-Arabino- Heptulosonate 7-Phosphate Synthase I181D Interface Mutant within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn1263

b:27.0
occ:0.50
OE2 B:GLU227 2.0 27.1 1.0
OD2 B:ASP238 2.4 40.5 1.0
SG B:CYS31 2.5 23.9 1.0
NE2 B:HIS201 2.6 26.8 1.0
CD B:GLU227 3.0 22.0 1.0
CE1 B:HIS201 3.2 26.6 1.0
CB B:CYS31 3.3 19.6 1.0
OE1 B:GLU227 3.4 22.3 1.0
CG B:ASP238 3.5 39.3 1.0
CL B:CL1267 3.7 29.1 0.4
CD2 B:HIS201 3.8 25.1 1.0
O B:HOH2061 3.9 40.7 1.0
CA B:CYS31 4.1 18.2 1.0
CB B:ASP238 4.1 37.3 1.0
NE2 B:GLN241 4.2 29.6 1.0
NZ B:LYS60 4.2 28.5 0.6
CG B:GLU227 4.3 18.7 1.0
ND1 B:HIS201 4.4 24.3 1.0
OD1 B:ASP238 4.6 38.6 1.0
NH2 B:ARG55 4.6 22.6 0.5
CG B:HIS201 4.7 22.0 1.0
NH2 B:ARG55 4.7 24.5 0.5
C B:CYS31 5.0 17.6 1.0
CE B:LYS60 5.0 29.0 0.6

Reference:

A.R.Nazmi, L.R.Schofield, R.C.J.Dobson, G.B.Jameson, E.J.Parker. Destabilization of the Homotetrameric Assembly of 3-Deoxy-D-Arabino-Heptulosonate 7-Phosphate Synthase From the Hyperthermophile Pyrococcus Furiosus Enhances Enzymatic Activity J.Mol.Biol. V. 426 656 2014.
ISSN: ISSN 0022-2836
PubMed: 24239948
DOI: 10.1016/J.JMB.2013.11.008
Page generated: Tue Dec 15 04:18:36 2020

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