Manganese in PDB 4b5h: Substate Bound Inactive Mutant of Neisseria Ap Endonuclease in Presence of Metal Ions

All present enzymatic activity of Substate Bound Inactive Mutant of Neisseria Ap Endonuclease in Presence of Metal Ions:
3.1.11.2;

The structure of Substate Bound Inactive Mutant of Neisseria Ap Endonuclease in Presence of Metal Ions, PDB code: 4b5h was solved by D.Lu, J.Silhan, J.T.Macdonald, E.P.Carpenter, K.Jensen, C.M.Tang, G.S.Baldwin, P.S.Freemont, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	31.07 / 3.05
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	68.907, 68.907, 120.968, 90.00, 90.00, 90.00
R / Rfree (%)	18.5 / 29.5

The binding sites of Manganese atom in the Substate Bound Inactive Mutant of Neisseria Ap Endonuclease in Presence of Metal Ions (pdb code 4b5h). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Substate Bound Inactive Mutant of Neisseria Ap Endonuclease in Presence of Metal Ions, PDB code: 4b5h:

Manganese binding site 1 out of 1 in the Substate Bound Inactive Mutant of Neisseria Ap Endonuclease in Presence of Metal Ions


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Substate Bound Inactive Mutant of Neisseria Ap Endonuclease in Presence of Metal Ions within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1260 b:88.8 occ:1.00 OD1 A:ASN10 2.5 72.2 1.0 OE1 A:GLU36 2.5 92.6 1.0 OE2 A:GLU36 3.0 0.2 1.0 CD A:GLU36 3.1 90.0 1.0 CG A:ASN10 3.2 64.1 1.0 OP1 U:3DR36 3.4 0.2 1.0 OD2 A:ASP246 3.7 68.3 1.0 ND2 A:ASN10 3.8 48.6 1.0 OD1 A:ASN8 3.9 57.9 1.0 CB A:ASN10 4.1 58.8 1.0 P U:3DR36 4.2 0.2 1.0 OP2 U:3DR36 4.3 0.6 1.0 OH A:TYR109 4.4 68.0 1.0 OD1 A:ASP246 4.4 59.8 1.0 O3' U:DC35 4.4 69.3 1.0 CG A:ASP246 4.5 53.2 1.0 NE2 A:HIS247 4.5 47.5 1.0 CA A:ASN10 4.5 61.0 1.0 CG A:GLU36 4.6 67.0 1.0 CD2 A:HIS247 4.6 44.7 1.0 NZ A:LYS38 4.6 33.5 1.0 C4' U:DC35 4.7 79.5 1.0 CE A:LYS38 4.8 45.0 1.0 CG A:ASN8 4.9 53.0 1.0

D.Lu, J.Silhan, J.T.Macdonald, E.P.Carpenter, K.Jensen, C.M.Tang, G.S.Baldwin, P.S.Freemont. Structural Basis For the Recognition and Cleavage of Abasic Dna in Neisseria Meningitidis. Proc. Natl. Acad. Sci. V. 109 16852 2012U.S.A..
ISSN: ESSN 1091-6490
PubMed: 23035246
DOI: 10.1073/PNAS.1206563109