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Manganese in PDB 3zgf: Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group)

Enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group)

All present enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group):
2.4.1.37; 2.4.1.40;

Protein crystallography data

The structure of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group), PDB code: 3zgf was solved by R.Jorgensen, G.O.Batot, O.Hindsgaul, H.Tanaka, S.Perez, A.Imberty, C.Breton, A.Royant, M.M.Palcic, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.98 / 1.70
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 52.840, 78.450, 155.130, 90.00, 90.00, 90.00
R / Rfree (%) 18.2 / 21.7

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group) (pdb code 3zgf). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group), PDB code: 3zgf:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 3zgf

Go back to Manganese Binding Sites List in 3zgf
Manganese binding site 1 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group) within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn1

b:27.0
occ:1.00
 O A:HOH2001 2.1 26.8 1.0
OD2 A:ASP211 2.2 29.7 1.0
O1A A:UDP1355 2.2 32.1 1.0
OD2 A:ASP213 2.2 31.2 1.0
OD1 A:ASP213 2.3 25.7 1.0
O3B A:UDP1355 2.4 26.3 1.0
CG A:ASP213 2.6 24.7 1.0
CG A:ASP211 3.2 24.8 1.0
PB A:UDP1355 3.5 39.0 1.0
PA A:UDP1355 3.5 34.2 1.0
CB A:ASP211 3.6 17.0 1.0
O3' A:UDP1355 3.8 24.1 1.0
O A:HOH2179 3.9 43.4 1.0
O3A A:UDP1355 3.9 44.6 1.0
O1B A:UDP1355 3.9 33.2 1.0
CB A:ASP213 4.1 28.3 1.0
NZ A:LYS346 4.1 63.8 1.0
OD1 A:ASP211 4.3 20.9 1.0
C5' A:UDP1355 4.4 22.7 1.0
O5' A:UDP1355 4.5 23.8 1.0
O2A A:UDP1355 4.5 37.5 1.0
C3' A:UDP1355 4.6 21.4 1.0
O2B A:UDP1355 4.8 49.9 1.0
O A:ASP213 4.8 22.0 1.0
CE A:LYS346 4.8 67.4 1.0
N A:ASP213 4.9 22.5 1.0
SD A:MET214 4.9 23.7 1.0
CA A:ASP213 4.9 26.5 1.0
O A:HOH2178 4.9 34.5 1.0
CA A:ASP211 5.0 19.2 1.0
C4' A:UDP1355 5.0 25.0 1.0

Manganese binding site 2 out of 2 in 3zgf

Go back to Manganese Binding Sites List in 3zgf
Manganese binding site 2 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with in Complex with Npe Caged Udp-Gal (P2(1)2(1)2(1) Space Group) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn1

b:25.7
occ:1.00
 O B:HOH2001 2.2 27.4 1.0
OD1 B:ASP213 2.2 23.1 1.0
OD2 B:ASP211 2.3 23.8 1.0
OAL B:IUG452 2.3 29.2 1.0
OD2 B:ASP213 2.3 29.9 1.0
OAE B:IUG452 2.3 34.6 1.0
CG B:ASP213 2.6 25.0 1.0
CG B:ASP211 3.2 22.0 1.0
PBT B:IUG452 3.5 43.8 1.0
CB B:ASP211 3.6 20.0 1.0
PBS B:IUG452 3.6 24.0 1.0
O3' B:IUG452 3.8 20.8 1.0
OBC B:IUG452 3.8 28.3 1.0
C1 B:IUG452 3.9 50.0 1.0
CB B:ASP213 4.1 25.2 1.0
O1 B:IUG452 4.2 49.5 1.0
OD1 B:ASP211 4.4 19.2 1.0
C5' B:IUG452 4.5 26.3 1.0
O5 B:IUG452 4.5 41.1 1.0
O B:HOH2249 4.5 43.8 1.0
O5' B:IUG452 4.6 24.1 1.0
C3' B:IUG452 4.6 19.1 1.0
OAD B:IUG452 4.7 33.1 1.0
O B:HOH2146 4.7 28.4 1.0
SD B:MET214 4.8 20.7 1.0
N B:ASP213 4.8 19.9 1.0
OAM B:IUG452 4.9 50.7 1.0
CA B:ASP213 4.9 23.6 1.0
CA B:ASP211 4.9 18.1 1.0
O B:ASP213 5.0 22.8 1.0
O2 B:IUG452 5.0 49.7 1.0
C2 B:IUG452 5.0 53.0 1.0

Reference:

R.Jorgensen, G.Batot, K.Mannerstedt, A.Imberty, C.Breton, O.Hindsgaul, A.Royant, M.M.Palcic. Structures of A Human Blood Group Glycosyltransferase in Complex with A Photo-Activatable Udp-Gal Derivative Reveal Two Different Binding Conformations Acta Crystallogr.,Sect.F V. 70 1015 2014.
ISSN: ESSN 1744-3091
PubMed: 25084373
DOI: 10.1107/S2053230X1401259X
Page generated: Tue Dec 15 04:17:52 2020

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