Manganese in PDB 3wzh: Crystal Structure of AFCSX3

The structure of Crystal Structure of AFCSX3, PDB code: 3wzh was solved by Y.A.Yuan, X.Yan, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	22.88 / 3.31
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	86.726, 86.726, 112.529, 90.00, 90.00, 120.00
R / Rfree (%)	22.1 / 25.7

The binding sites of Manganese atom in the Crystal Structure of AFCSX3 (pdb code 3wzh). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 5 binding sites of Manganese where determined in the Crystal Structure of AFCSX3, PDB code: 3wzh:
Jump to Manganese binding site number: 1; 2; 3; 4; 5;

Manganese binding site 1 out of 5 in the Crystal Structure of AFCSX3


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of AFCSX3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn201 b:33.1 occ:1.00 NZ A:LYS58 3.1 31.1 1.0 OE2 B:GLU85 3.4 26.5 1.0 NE2 A:HIS57 3.5 30.2 1.0 CD2 A:HIS57 4.4 28.3 1.0 CE1 A:HIS57 4.4 31.4 1.0 CD B:GLU85 4.4 25.7 1.0 CE A:LYS58 4.5 29.4 1.0 OE1 B:GLU85 4.6 28.0 1.0 CD A:LYS58 4.8 28.7 1.0

Manganese binding site 2 out of 5 in the Crystal Structure of AFCSX3


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of AFCSX3 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn202 b:60.2 occ:1.00 CE1 A:HIS80 3.6 56.1 1.0 NE2 A:HIS80 3.7 53.1 1.0 ND1 A:HIS60 3.9 37.0 1.0 NE2 B:HIS60 3.9 38.0 1.0 CE1 B:HIS57 4.0 31.7 1.0 CD2 B:HIS60 4.3 35.5 1.0 O A:HIS60 4.3 36.9 1.0 CE1 B:HIS60 4.5 36.6 1.0 OE1 A:GLN78 4.7 36.7 1.0 ND1 B:HIS57 4.7 29.5 1.0 ND1 A:HIS80 4.7 56.6 1.0 CE1 A:HIS60 4.8 37.8 1.0 CB A:HIS60 4.8 32.4 1.0 CG A:HIS60 4.8 33.8 1.0 CD2 A:HIS80 4.9 51.5 1.0 NE2 B:HIS57 5.0 31.7 1.0

Manganese binding site 3 out of 5 in the Crystal Structure of AFCSX3


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of AFCSX3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn201 b:44.2 occ:1.00 NH1 B:ARG84 3.5 30.3 1.0 CD B:ARG84 3.8 25.6 1.0 N B:GLU85 3.8 21.1 1.0 CD B:GLU85 3.9 25.7 1.0 OE1 B:GLU85 3.9 28.0 1.0 CB B:GLU85 4.0 22.7 1.0 CG B:GLU85 4.0 22.8 1.0 OE2 B:GLU85 4.3 26.5 1.0 CA B:ARG84 4.3 21.9 1.0 CB B:ARG84 4.3 23.1 1.0 CZ B:ARG84 4.4 29.8 1.0 NE B:ARG84 4.5 27.6 1.0 CA B:GLU85 4.6 20.9 1.0 C B:ARG84 4.6 20.6 1.0 CG B:ARG84 4.7 24.0 1.0 OG B:SER79 5.0 27.0 1.0

Manganese binding site 4 out of 5 in the Crystal Structure of AFCSX3


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Crystal Structure of AFCSX3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn202 b:49.6 occ:1.00 NZ B:LYS58 3.2 35.2 1.0 NE2 B:HIS57 3.8 31.7 1.0 CE B:LYS58 4.5 33.0 1.0 CE1 B:HIS57 4.6 31.7 1.0 CD2 B:HIS57 4.7 29.5 1.0 CD B:LYS58 4.9 30.5 1.0

Manganese binding site 5 out of 5 in the Crystal Structure of AFCSX3


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of Crystal Structure of AFCSX3 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn203 b:61.6 occ:1.00 CE1 B:HIS80 3.4 42.3 1.0 O B:HOH312 3.6 8.4 1.0 NE2 B:HIS80 3.6 40.6 1.0 CE1 A:HIS60 3.7 37.8 1.0 CD2 B:HIS60 3.9 35.5 1.0 CE1 A:HIS57 4.0 31.4 1.0 ND1 A:HIS60 4.2 37.0 1.0 O B:HIS60 4.2 31.4 1.0 NE2 A:HIS60 4.4 35.5 1.0 ND1 B:HIS80 4.6 41.6 1.0 CG B:HIS60 4.6 33.1 1.0 ND1 A:HIS57 4.6 30.2 1.0 CB B:HIS60 4.6 30.2 1.0 NE2 B:GLN78 4.8 29.6 1.0 CD2 B:HIS80 4.9 38.6 1.0 NE2 B:HIS60 5.0 38.0 1.0

X.Yan, W.Guo, Y.A.Yuan. Crystal Structures of Crispr-Associated CSX3 Reveal A Manganese-Dependent Deadenylation Exoribonuclease Rna Biol. 2015.
ISSN: ESSN 1555-8584
PubMed: 26106927
DOI: 10.1080/15476286.2015.1051300