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Manganese in PDB 3v0q: Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor

Enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor

All present enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor:
2.4.1.37; 2.4.1.40;

Protein crystallography data

The structure of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor, PDB code: 3v0q was solved by M.M.Palcic, R.Jorgensen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.94 / 1.80
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 78.100, 153.670, 52.500, 90.00, 90.00, 90.00
R / Rfree (%) 15.1 / 19.1

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor (pdb code 3v0q). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor, PDB code: 3v0q:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 3v0q

Go back to Manganese Binding Sites List in 3v0q
Manganese binding site 1 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn401

b:16.4
occ:1.00
 OD2 A:ASP211 2.2 13.5 1.0
O2A A:UDP402 2.2 15.4 1.0
O2B A:UDP402 2.3 21.1 1.0
OD1 A:ASP213 2.3 14.1 1.0
O A:HOH619 2.3 17.3 1.0
OD2 A:ASP213 2.5 15.1 1.0
CG A:ASP213 2.7 13.8 1.0
CG A:ASP211 3.2 11.5 1.0
PB A:UDP402 3.4 25.7 1.0
PA A:UDP402 3.5 16.5 1.0
CB A:ASP211 3.5 9.5 1.0
O3A A:UDP402 3.6 18.2 1.0
O A:HOH684 3.7 30.4 1.0
O3' A:UDP402 3.8 15.3 1.0
O A:HOH606 4.1 25.7 1.0
O1B A:UDP402 4.1 23.5 1.0
NZ A:LYS346 4.1 23.0 1.0
C5' A:UDP402 4.2 12.8 1.0
CB A:ASP213 4.2 11.8 1.0
OD1 A:ASP211 4.3 9.3 1.0
O5' A:UDP402 4.4 12.2 1.0
C3' A:UDP402 4.5 15.2 1.0
O1A A:UDP402 4.5 18.9 1.0
O3B A:UDP402 4.6 26.1 1.0
C4' A:UDP402 4.8 14.8 1.0
O A:HOH613 4.8 17.8 1.0
O A:ASP213 4.9 11.1 1.0
N A:ASP213 4.9 11.6 1.0
SD A:MET214 4.9 11.9 1.0
CA A:ASP211 4.9 8.2 1.0
CE A:LYS346 5.0 24.4 1.0

Manganese binding site 2 out of 2 in 3v0q

Go back to Manganese Binding Sites List in 3v0q
Manganese binding site 2 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp and H-Antigen Acceptor within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn401

b:12.8
occ:1.00
 OD2 B:ASP211 2.1 11.7 1.0
O2B B:UDP402 2.1 14.4 1.0
O B:HOH553 2.2 8.8 1.0
O2A B:UDP402 2.2 13.3 1.0
OD1 B:ASP213 2.3 12.8 1.0
OD2 B:ASP213 2.5 14.8 1.0
CG B:ASP213 2.7 12.9 1.0
CG B:ASP211 3.2 11.3 1.0
PB B:UDP402 3.3 16.7 1.0
PA B:UDP402 3.4 13.6 1.0
CB B:ASP211 3.5 8.9 1.0
O3A B:UDP402 3.5 14.8 1.0
O3' B:UDP402 3.8 11.6 1.0
O B:HOH544 3.8 19.8 1.0
O1B B:UDP402 3.9 18.1 1.0
O B:HOH669 4.1 27.9 1.0
NZ B:LYS346 4.1 16.6 1.0
C5' B:UDP402 4.2 9.3 1.0
CB B:ASP213 4.2 12.4 1.0
OD1 B:ASP211 4.3 11.5 1.0
O5' B:UDP402 4.3 9.7 1.0
O1A B:UDP402 4.4 16.3 1.0
C3' B:UDP402 4.5 10.9 1.0
O3B B:UDP402 4.5 19.0 1.0
O B:HOH551 4.8 14.7 1.0
C4' B:UDP402 4.8 10.0 1.0
O B:HOH612 4.9 15.2 1.0
N B:ASP213 4.9 10.3 1.0
CA B:ASP211 4.9 7.6 1.0
SD B:MET214 4.9 12.7 1.0
O B:ASP213 5.0 10.9 1.0

Reference:

R.Jrgensen, T.Pesnot, H.J.Lee, M.M.Palcic, G.K.Wagner. Base-Modified Donor Analogues Reveal Novel Dynamic Features of A Glycosyltransferase. J.Biol.Chem. V. 288 26201 2013.
ISSN: ISSN 0021-9258
PubMed: 23836908
DOI: 10.1074/JBC.M113.465963
Page generated: Sat Oct 5 18:19:37 2024

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