Manganese in PDB 3s1s: Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Bpusi

The structure of Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Bpusi, PDB code: 3s1s was solved by B.W.Shen, D.Xu, S.-H.Chan, Y.Zheng, Y.Zhu, S.-Y.Xu, B.L.Stoddard, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.35
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	213.472, 215.732, 73.710, 90.00, 90.00, 90.00
R / Rfree (%)	21.2 / 26.8

The structure of Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Bpusi also contains other interesting chemical elements:

Iodine

(I)

38 atoms

The binding sites of Manganese atom in the Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Bpusi (pdb code 3s1s). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Bpusi, PDB code: 3s1s:

Manganese binding site 1 out of 1 in the Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Bpusi


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Bpusi within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn930 b:27.7 occ:1.00 O A:HOH1218 1.9 38.8 1.0 O A:VAL77 2.1 27.7 1.0 OD2 A:ASP60 2.1 31.4 1.0 OE2 A:GLU76 2.3 34.0 1.0 O A:HOH1129 2.4 21.6 1.0 OE2 A:GLU21 2.4 26.9 1.0 CG A:ASP60 3.1 30.9 1.0 C A:VAL77 3.2 27.8 1.0 CD A:GLU21 3.2 27.2 1.0 OE1 A:GLU21 3.2 26.1 1.0 CD A:GLU76 3.3 34.7 1.0 OD1 A:ASP60 3.3 29.6 1.0 N A:VAL77 3.6 28.3 1.0 CA A:VAL77 3.8 27.8 1.0 OE1 A:GLU76 3.9 35.9 1.0 CB A:VAL77 4.1 28.2 1.0 NE2 A:HIS26 4.2 26.3 1.0 NZ A:LYS78 4.2 33.6 1.0 CG A:GLU76 4.3 31.5 1.0 CE A:LYS78 4.3 31.7 1.0 N A:LYS78 4.3 27.3 1.0 CB A:ASP60 4.4 31.3 1.0 OE2 A:GLU533 4.5 41.2 1.0 O A:HOH1063 4.5 35.0 1.0 OE1 A:GLU533 4.5 42.2 1.0 CD A:GLU533 4.6 39.6 1.0 CG A:GLU21 4.6 27.9 1.0 C A:GLU76 4.7 28.2 1.0 CA A:LYS78 4.7 27.2 1.0 CD A:LYS78 4.8 29.4 1.0 CE1 A:HIS26 4.8 25.3 1.0

B.W.Shen, D.Xu, S.H.Chan, Y.Zheng, Z.Zhu, S.Y.Xu, B.L.Stoddard. Characterization and Crystal Structure of the Type Iig Restriction Endonuclease Rm.Bpusi. Nucleic Acids Res. V. 39 8223 2011.
ISSN: ISSN 0305-1048
PubMed: 21724614
DOI: 10.1093/NAR/GKR543