Manganese in PDB 3pvb: Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase

All present enzymatic activity of Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase:
2.7.11.11;

The structure of Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase, PDB code: 3pvb was solved by A.J.Boettcher, J.Wu, C.Kim, J.Yang, J.Bruystens, N.Cheung, J.K.Pennypacker, D.A.Blumenthal, A.P.Kornev, S.S.Taylor, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	50.00 / 3.30
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	116.667, 116.667, 140.103, 90.00, 90.00, 120.00
R / Rfree (%)	24.2 / 29

The binding sites of Manganese atom in the Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase (pdb code 3pvb). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase, PDB code: 3pvb:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn401 b:18.1 occ:1.00 OD1 A:ASN171 1.8 13.9 1.0 N3B A:ANP400 2.0 29.6 1.0 O2A A:ANP400 2.2 25.5 1.0 O2G A:ANP400 2.5 30.8 1.0 CG A:ASN171 2.8 15.0 1.0 PG A:ANP400 2.8 31.9 1.0 OD2 A:ASP184 3.1 7.5 1.0 ND2 A:ASN171 3.2 16.3 1.0 PA A:ANP400 3.5 25.6 1.0 PB A:ANP400 3.5 28.6 1.0 CG A:ASP184 3.6 9.2 1.0 O3G A:ANP400 3.7 31.6 1.0 CB A:ASP184 3.8 11.7 1.0 CE A:LYS168 3.8 9.5 1.0 O3A A:ANP400 3.9 26.8 1.0 O1G A:ANP400 4.0 33.0 1.0 O3' A:ANP400 4.0 17.4 1.0 CB A:ASN171 4.1 15.2 1.0 NZ A:LYS168 4.1 9.4 1.0 O1A A:ANP400 4.2 23.5 1.0 O2B A:ANP400 4.3 31.3 1.0 OD1 A:ASP184 4.5 9.0 1.0 CA A:ASN171 4.5 16.8 1.0 O1B A:ANP400 4.6 28.1 1.0 O A:GLU170 4.6 14.5 1.0 MN A:MN402 4.6 7.3 1.0 O5' A:ANP400 4.7 24.5 1.0 C3' A:ANP400 4.7 22.1 1.0 N A:ASN171 4.7 16.4 1.0 C A:GLU170 4.8 14.4 1.0 C5' A:ANP400 4.9 23.7 1.0 OD2 A:ASP166 4.9 16.2 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn402 b:7.3 occ:1.00 O1G A:ANP400 2.0 33.0 1.0 OD1 A:ASP184 2.2 9.0 1.0 OD2 A:ASP184 2.6 7.5 1.0 CG A:ASP184 2.8 9.2 1.0 O2B A:ANP400 2.8 31.3 1.0 PG A:ANP400 3.2 31.9 1.0 N3B A:ANP400 3.3 29.6 1.0 PB A:ANP400 3.5 28.6 1.0 OD2 A:ASP166 3.6 16.2 1.0 O2G A:ANP400 3.9 30.8 1.0 CD1 A:PHE54 3.9 33.0 1.0 O1B A:ANP400 3.9 28.1 1.0 CE1 A:PHE187 4.0 11.2 1.0 CE1 A:PHE54 4.1 33.2 1.0 CB B:ALA97 4.2 8.8 1.0 CB A:ASP184 4.3 11.7 1.0 CD1 A:PHE187 4.3 9.8 1.0 O3G A:ANP400 4.4 31.6 1.0 MN A:MN401 4.6 18.1 1.0 CZ A:PHE187 4.6 11.3 1.0 CG A:ASP166 4.8 17.2 1.0 O A:ASP184 5.0 10.4 1.0 O3A A:ANP400 5.0 26.8 1.0

A.J.Boettcher, J.Wu, C.Kim, J.Yang, J.Bruystens, N.Cheung, J.K.Pennypacker, D.A.Blumenthal, A.P.Kornev, S.S.Taylor. Crystal Structure of (73-244)Ria:C Holoenzyme of Camp-Dependent Protein Kinase Structure V. 19 265 2011.
ISSN: ISSN 0969-2126
PubMed: 21300294
DOI: 10.1016/J.STR.2010.12.005