Manganese in PDB 3mwt: Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+

The structure of Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+, PDB code: 3mwt was solved by X.Qi, S.Lan, W.Wang, L.M.Schelde, H.Dong, G.Wallat, Y.Liang, H.Ly, C.Dong, Scottish Structural Proteomics Facility (Sspf), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	153.18 / 1.98
Space group	P 3
Cell size a, b, c (Å), α, β, γ (°)	176.880, 176.880, 56.469, 90.00, 90.00, 120.00
R / Rfree (%)	18.1 / 21.8

The structure of Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+ also contains other interesting chemical elements:

Zinc

(Zn)

3 atoms

The binding sites of Manganese atom in the Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+ (pdb code 3mwt). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+, PDB code: 3mwt:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in the Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn680 b:76.5 occ:1.00 OD1 A:ASP389 2.1 38.0 1.0 OD2 A:ASP533 2.3 40.3 1.0 OE2 A:GLU391 2.8 41.8 1.0 CG A:ASP389 3.1 33.4 1.0 CG A:ASP533 3.4 41.6 1.0 OD2 A:ASP389 3.5 33.6 1.0 CB A:ASP533 3.8 39.4 1.0 O A:ILE390 3.9 39.3 1.0 CD A:GLU391 3.9 45.5 1.0 O A:HOH588 4.2 42.0 1.0 CG A:GLU391 4.4 44.9 1.0 O A:CYS529 4.4 36.9 1.0 CB A:ASP389 4.4 30.2 1.0 OD1 A:ASP533 4.5 43.5 1.0 NH2 A:ARG492 4.5 40.9 1.0 CB A:HIS528 4.6 37.4 1.0 CA A:ALA530 4.6 32.2 1.0 C A:ILE390 4.7 36.9 1.0 C A:CYS529 4.8 34.9 1.0 N A:ALA530 4.8 30.3 1.0 CZ A:PHE537 4.9 39.5 1.0 CE2 A:PHE537 5.0 40.3 1.0 OE1 A:GLU391 5.0 66.9 1.0

Manganese binding site 2 out of 3 in the Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn680 b:54.8 occ:1.00 OD2 B:ASP533 2.1 31.1 1.0 OD1 B:ASP389 2.2 28.4 1.0 OE2 B:GLU391 2.3 32.7 1.0 CG B:ASP533 3.1 29.5 1.0 CG B:ASP389 3.2 30.6 1.0 CD B:GLU391 3.4 32.4 1.0 CB B:ASP533 3.5 31.8 1.0 OD2 B:ASP389 3.6 28.3 1.0 OE1 B:GLU391 3.7 29.8 1.0 O B:ILE390 3.9 32.3 1.0 OD1 B:ASP533 4.2 30.1 1.0 CB B:HIS528 4.5 31.9 1.0 CB B:ASP389 4.6 26.6 1.0 NH2 B:ARG492 4.6 39.4 1.0 CA B:ALA530 4.6 25.6 1.0 O B:CYS529 4.7 29.5 1.0 CG B:GLU391 4.7 31.1 1.0 C B:ILE390 4.8 27.8 1.0 CZ B:PHE537 4.8 34.4 1.0 CE2 B:PHE537 4.8 36.7 1.0 N B:ALA530 5.0 27.8 1.0 C B:CYS529 5.0 29.2 1.0 ND1 B:HIS528 5.0 38.0 1.0 N B:ILE390 5.0 29.0 1.0

Manganese binding site 3 out of 3 in the Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Lassa Fever Virus Nucleoprotein in Complex with MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn680 b:87.4 occ:1.00 OD2 C:ASP533 2.2 43.9 1.0 OE2 C:GLU391 2.3 49.6 1.0 OD1 C:ASP389 2.7 45.0 1.0 CG C:ASP533 3.3 42.8 1.0 CD C:GLU391 3.3 46.6 1.0 CG C:ASP389 3.4 46.7 1.0 OD2 C:ASP389 3.6 53.1 1.0 OE1 C:GLU391 3.7 42.4 1.0 CB C:ASP533 3.8 37.5 1.0 NH2 C:ARG492 4.1 57.0 1.0 O C:ILE390 4.1 45.7 1.0 OD1 C:ASP533 4.4 42.3 1.0 CB C:HIS528 4.6 50.0 1.0 CB C:ASP389 4.7 39.2 1.0 CG C:GLU391 4.7 42.6 1.0 O C:CYS529 4.8 37.9 1.0 CA C:ALA530 4.8 37.5 1.0 CE2 C:PHE537 4.8 40.6 1.0 CZ C:PHE537 4.9 39.1 1.0 ND1 C:HIS528 4.9 56.6 1.0 CG C:HIS528 5.0 52.4 1.0

X.Qi, S.Lan, W.Wang, L.M.Schelde, H.Dong, G.D.Wallat, H.Ly, Y.Liang, C.Dong. Cap Binding and Immune Evasion Revealed By Lassa Nucleoprotein Structure. Nature V. 468 779 2010.
ISSN: ISSN 0028-0836
PubMed: 21085117
DOI: 10.1038/NATURE09605