Atomistry » Manganese » PDB 3hw6-3ki9 » 3ioj
Atomistry »
  Manganese »
    PDB 3hw6-3ki9 »
      3ioj »

Manganese in PDB 3ioj: Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp

Enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp

All present enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp:
2.4.1.37; 2.4.1.40;

Protein crystallography data

The structure of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp, PDB code: 3ioj was solved by T.Pesnot, R.Jorgensen, M.M.Palcic, G.K.Wagner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 33.13 / 1.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 52.450, 77.536, 153.605, 90.00, 90.00, 90.00
R / Rfree (%) 16.3 / 19.3

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp (pdb code 3ioj). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp, PDB code: 3ioj:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 3ioj

Go back to Manganese Binding Sites List in 3ioj
Manganese binding site 1 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn406

b:22.8
occ:1.00
O3B A:UDP475 2.2 23.4 1.0
O1A A:UDP475 2.2 24.5 1.0
OD2 A:ASP211 2.2 22.4 1.0
OD1 A:ASP213 2.2 21.6 1.0
O A:HOH513 2.3 27.0 1.0
OD2 A:ASP213 2.5 28.6 1.0
CG A:ASP213 2.7 26.1 1.0
CG A:ASP211 3.2 18.7 1.0
PB A:UDP475 3.3 27.3 1.0
PA A:UDP475 3.3 24.6 1.0
O3A A:UDP475 3.5 21.1 1.0
CB A:ASP211 3.6 17.9 1.0
O3' A:UDP475 3.8 23.5 1.0
O2B A:UDP475 4.0 28.3 1.0
NZ A:LYS346 4.1 35.7 1.0
CB A:ASP213 4.2 22.9 1.0
C5' A:UDP475 4.3 23.4 1.0
O5' A:UDP475 4.3 19.6 1.0
OD1 A:ASP211 4.3 19.8 1.0
O2A A:UDP475 4.4 27.6 1.0
O1B A:UDP475 4.4 29.6 1.0
C3' A:UDP475 4.5 22.9 1.0
CE A:LYS346 4.8 41.1 1.0
O A:HOH533 4.8 36.8 1.0
O A:HOH369 4.9 22.7 1.0
O A:ASP213 4.9 17.6 1.0
C4' A:UDP475 4.9 24.1 1.0
SD A:MET214 4.9 20.8 1.0
N A:ASP213 5.0 16.0 1.0
CA A:ASP211 5.0 16.5 1.0
CB A:ALA268 5.0 15.6 1.0

Manganese binding site 2 out of 2 in 3ioj

Go back to Manganese Binding Sites List in 3ioj
Manganese binding site 2 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn406

b:24.9
occ:1.00
O1A B:UDP475 2.2 25.0 1.0
O3B B:UDP475 2.2 29.5 1.0
OD2 B:ASP211 2.2 17.0 1.0
OD1 B:ASP213 2.3 19.1 1.0
O B:HOH454 2.3 26.3 1.0
OD2 B:ASP213 2.6 24.9 1.0
CG B:ASP213 2.8 21.2 1.0
CG B:ASP211 3.2 17.7 1.0
PB B:UDP475 3.3 30.7 1.0
PA B:UDP475 3.4 26.2 1.0
CB B:ASP211 3.5 14.9 1.0
O3A B:UDP475 3.6 24.7 1.0
O3' B:UDP475 3.8 22.9 1.0
O2B B:UDP475 4.1 31.5 1.0
NZ B:LYS346 4.2 43.4 1.0
C5' B:UDP475 4.3 24.1 1.0
OD1 B:ASP211 4.3 16.6 1.0
CB B:ASP213 4.3 17.6 1.0
O5' B:UDP475 4.4 22.1 1.0
O B:HOH524 4.4 36.3 1.0
O2A B:UDP475 4.4 26.7 1.0
O1B B:UDP475 4.4 31.7 1.0
C3' B:UDP475 4.6 21.5 1.0
O B:HOH383 4.8 28.0 1.0
CE B:LYS346 4.9 44.9 1.0
O B:HOH569 4.9 29.4 1.0
O B:ASP213 4.9 16.4 1.0
SD B:MET214 4.9 17.8 1.0
C4' B:UDP475 4.9 21.4 1.0
CB B:ALA268 4.9 12.8 1.0
CA B:ASP211 5.0 15.8 1.0
N B:ASP213 5.0 16.9 1.0

Reference:

T.Pesnot, R.Jorgensen, M.M.Palcic, G.K.Wagner. Structural and Mechanistic Basis For A New Mode of Glycosyltransferase Inhibition. Nat.Chem.Biol. V. 6 321 2010.
ISSN: ISSN 1552-4450
PubMed: 20364127
DOI: 10.1038/NCHEMBIO.343
Page generated: Tue Dec 15 04:10:56 2020

Last articles

Zn in 8WB0
Zn in 8WAX
Zn in 8WAU
Zn in 8WAZ
Zn in 8WAY
Zn in 8WAV
Zn in 8WAW
Zn in 8WAT
Zn in 8W7M
Zn in 8WD3
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy