Atomistry » Manganese » PDB 3hw6-3ki9 » 3ioj
Atomistry »
  Manganese »
    PDB 3hw6-3ki9 »
      3ioj »

Manganese in PDB 3ioj: Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp

Enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp

All present enzymatic activity of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp:
2.4.1.37; 2.4.1.40;

Protein crystallography data

The structure of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp, PDB code: 3ioj was solved by T.Pesnot, R.Jorgensen, M.M.Palcic, G.K.Wagner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 33.13 / 1.65
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 52.450, 77.536, 153.605, 90.00, 90.00, 90.00
R / Rfree (%) 16.3 / 19.3

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp (pdb code 3ioj). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp, PDB code: 3ioj:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in 3ioj

Go back to Manganese Binding Sites List in 3ioj
Manganese binding site 1 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn406

b:22.8
occ:1.00
 O3B A:UDP475 2.2 23.4 1.0
O1A A:UDP475 2.2 24.5 1.0
OD2 A:ASP211 2.2 22.4 1.0
OD1 A:ASP213 2.2 21.6 1.0
O A:HOH513 2.3 27.0 1.0
OD2 A:ASP213 2.5 28.6 1.0
CG A:ASP213 2.7 26.1 1.0
CG A:ASP211 3.2 18.7 1.0
PB A:UDP475 3.3 27.3 1.0
PA A:UDP475 3.3 24.6 1.0
O3A A:UDP475 3.5 21.1 1.0
CB A:ASP211 3.6 17.9 1.0
O3' A:UDP475 3.8 23.5 1.0
O2B A:UDP475 4.0 28.3 1.0
NZ A:LYS346 4.1 35.7 1.0
CB A:ASP213 4.2 22.9 1.0
C5' A:UDP475 4.3 23.4 1.0
O5' A:UDP475 4.3 19.6 1.0
OD1 A:ASP211 4.3 19.8 1.0
O2A A:UDP475 4.4 27.6 1.0
O1B A:UDP475 4.4 29.6 1.0
C3' A:UDP475 4.5 22.9 1.0
CE A:LYS346 4.8 41.1 1.0
O A:HOH533 4.8 36.8 1.0
O A:HOH369 4.9 22.7 1.0
O A:ASP213 4.9 17.6 1.0
C4' A:UDP475 4.9 24.1 1.0
SD A:MET214 4.9 20.8 1.0
N A:ASP213 5.0 16.0 1.0
CA A:ASP211 5.0 16.5 1.0
CB A:ALA268 5.0 15.6 1.0

Manganese binding site 2 out of 2 in 3ioj

Go back to Manganese Binding Sites List in 3ioj
Manganese binding site 2 out of 2 in the Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the Fucosylgalactoside Alpha N- Acetylgalactosaminyltransferase (Gta, Cisab Mutant L266G, G268A) in Complex with Udp within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn406

b:24.9
occ:1.00
 O1A B:UDP475 2.2 25.0 1.0
O3B B:UDP475 2.2 29.5 1.0
OD2 B:ASP211 2.2 17.0 1.0
OD1 B:ASP213 2.3 19.1 1.0
O B:HOH454 2.3 26.3 1.0
OD2 B:ASP213 2.6 24.9 1.0
CG B:ASP213 2.8 21.2 1.0
CG B:ASP211 3.2 17.7 1.0
PB B:UDP475 3.3 30.7 1.0
PA B:UDP475 3.4 26.2 1.0
CB B:ASP211 3.5 14.9 1.0
O3A B:UDP475 3.6 24.7 1.0
O3' B:UDP475 3.8 22.9 1.0
O2B B:UDP475 4.1 31.5 1.0
NZ B:LYS346 4.2 43.4 1.0
C5' B:UDP475 4.3 24.1 1.0
OD1 B:ASP211 4.3 16.6 1.0
CB B:ASP213 4.3 17.6 1.0
O5' B:UDP475 4.4 22.1 1.0
O B:HOH524 4.4 36.3 1.0
O2A B:UDP475 4.4 26.7 1.0
O1B B:UDP475 4.4 31.7 1.0
C3' B:UDP475 4.6 21.5 1.0
O B:HOH383 4.8 28.0 1.0
CE B:LYS346 4.9 44.9 1.0
O B:HOH569 4.9 29.4 1.0
O B:ASP213 4.9 16.4 1.0
SD B:MET214 4.9 17.8 1.0
C4' B:UDP475 4.9 21.4 1.0
CB B:ALA268 4.9 12.8 1.0
CA B:ASP211 5.0 15.8 1.0
N B:ASP213 5.0 16.9 1.0

Reference:

T.Pesnot, R.Jorgensen, M.M.Palcic, G.K.Wagner. Structural and Mechanistic Basis For A New Mode of Glycosyltransferase Inhibition. Nat.Chem.Biol. V. 6 321 2010.
ISSN: ISSN 1552-4450
PubMed: 20364127
DOI: 10.1038/NCHEMBIO.343
Page generated: Sat Oct 5 16:35:40 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy