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Manganese in PDB 3gbr: Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus

Enzymatic activity of Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus

All present enzymatic activity of Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus:
2.4.2.18;

Protein crystallography data

The structure of Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus, PDB code: 3gbr was solved by M.Bruning, S.Schlee, M.Deuss, A.Ivens, O.Mayans, R.Sterner, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 20.00 / 2.25
Space group P 1 21 1
Cell size a, b, c (Å), α, β, γ (°) 61.945, 65.993, 84.262, 90.00, 98.81, 90.00
R / Rfree (%) 19.2 / 26

Manganese Binding Sites:

The binding sites of Manganese atom in the Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus (pdb code 3gbr). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 4 binding sites of Manganese where determined in the Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus, PDB code: 3gbr:
Jump to Manganese binding site number: 1; 2; 3; 4;

Manganese binding site 1 out of 4 in 3gbr

Go back to Manganese Binding Sites List in 3gbr
Manganese binding site 1 out of 4 in the Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn501

b:46.5
occ:1.00
 OD1 A:ASP223 2.4 31.0 1.0
O A:HOH443 2.4 34.0 1.0
OE1 A:GLU224 2.6 23.4 1.0
CG A:ASP223 3.3 26.4 1.0
CD A:GLU224 3.4 33.9 1.0
CG A:GLU224 3.4 18.7 1.0
OD2 A:ASP223 3.4 28.5 1.0
O1B A:PRP401 3.5 26.1 1.0
MN A:MN502 3.6 27.8 1.0
O A:HOH425 4.0 31.8 1.0
O A:HOH395 4.0 27.0 1.0
OG1 A:THR87 4.0 39.2 1.0
O3 A:PRP401 4.2 24.8 1.0
O A:HOH444 4.4 17.4 1.0
O2B A:PRP401 4.5 22.7 1.0
PB A:PRP401 4.5 31.5 1.0
OE2 A:GLU224 4.5 38.8 1.0
O A:ASP223 4.6 25.1 1.0
CB A:ASP223 4.7 28.8 1.0
C A:ASP223 4.8 25.6 1.0
CB A:GLU224 4.9 24.8 1.0
N A:ASP223 4.9 28.3 1.0

Manganese binding site 2 out of 4 in 3gbr

Go back to Manganese Binding Sites List in 3gbr
Manganese binding site 2 out of 4 in the Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Mn502

b:27.8
occ:1.00
 O1B A:PRP401 1.4 26.1 1.0
O2A A:PRP401 2.1 25.0 1.0
O A:HOH444 2.2 17.4 1.0
OE1 A:GLU224 2.2 23.4 1.0
OG A:SER91 2.4 28.8 1.0
PB A:PRP401 2.9 31.5 1.0
CB A:SER91 3.0 21.9 1.0
CD A:GLU224 3.1 33.9 1.0
PA A:PRP401 3.2 24.2 1.0
OE2 A:GLU224 3.3 38.8 1.0
O3A A:PRP401 3.4 22.4 1.0
MN A:MN501 3.6 46.5 1.0
O1 A:PRP401 3.7 28.5 1.0
O3B A:PRP401 3.8 33.3 1.0
N A:GLY79 4.0 28.4 1.0
O2B A:PRP401 4.0 22.7 1.0
N A:SER91 4.0 23.4 1.0
CA A:SER91 4.1 25.5 1.0
O A:HOH443 4.3 34.0 1.0
OD2 A:ASP223 4.4 28.5 1.0
CG A:GLU224 4.4 18.7 1.0
O A:ASP223 4.5 25.1 1.0
CA A:GLY79 4.5 24.1 1.0
CG1 A:VAL90 4.5 21.6 1.0
O1A A:PRP401 4.6 24.6 1.0
C A:ALA78 4.6 26.1 1.0
CA A:ALA78 4.6 23.1 1.0
OD1 A:ASP223 4.6 31.0 1.0
CG A:ASP223 4.7 26.4 1.0

Manganese binding site 3 out of 4 in 3gbr

Go back to Manganese Binding Sites List in 3gbr
Manganese binding site 3 out of 4 in the Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn503

b:20.2
occ:1.00
 O1B B:PRP402 1.9 16.6 1.0
OE1 B:GLU224 2.0 17.4 1.0
O2A B:PRP402 2.1 21.4 1.0
O B:HOH481 2.2 15.9 1.0
OG B:SER91 2.3 22.7 1.0
O B:HOH484 2.5 18.4 1.0
CD B:GLU224 3.0 14.4 1.0
PA B:PRP402 3.2 18.6 1.0
CB B:SER91 3.2 17.4 1.0
PB B:PRP402 3.2 23.5 1.0
OE2 B:GLU224 3.4 22.9 1.0
O3A B:PRP402 3.5 19.3 1.0
O1 B:PRP402 3.7 19.4 1.0
MN B:MN504 3.9 69.3 1.0
N B:GLY79 4.0 11.6 1.0
O2B B:PRP402 4.1 20.1 1.0
N B:SER91 4.1 16.4 1.0
O B:ASP223 4.2 19.8 1.0
CA B:SER91 4.3 13.9 1.0
CG B:GLU224 4.3 15.1 1.0
O B:HOH374 4.4 24.1 1.0
O3B B:PRP402 4.4 15.4 1.0
OD2 B:ASP223 4.4 24.5 1.0
CA B:GLY79 4.5 16.1 1.0
O B:HOH482 4.5 23.7 1.0
O B:HOH410 4.5 28.7 1.0
O1A B:PRP402 4.6 19.5 1.0
C B:ALA78 4.6 12.6 1.0
CA B:ALA78 4.7 19.0 1.0
CG1 B:VAL90 4.7 14.6 1.0
CG B:ASP223 4.8 17.4 1.0
OD1 B:ASP223 4.8 19.8 1.0

Manganese binding site 4 out of 4 in 3gbr

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Manganese binding site 4 out of 4 in the Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Anthranilate Phosphoribosyl-Transferase (Trpd) Double Mutant D83G F149S From S. Solfataricus within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Mn504

b:69.3
occ:1.00
 O B:HOH374 2.7 24.1 1.0
OD1 B:ASP223 2.8 19.8 1.0
OE1 B:GLU224 2.8 17.4 1.0
O B:HOH484 3.0 18.4 1.0
CG B:GLU224 3.3 15.1 1.0
O B:HOH444 3.4 19.5 1.0
CD B:GLU224 3.5 14.4 1.0
CG B:ASP223 3.7 17.4 1.0
MN B:MN503 3.9 20.2 1.0
OD2 B:ASP223 4.0 24.5 1.0
O1B B:PRP402 4.1 16.6 1.0
O B:HOH388 4.2 34.4 1.0
O B:HOH447 4.4 36.4 1.0
O B:ASP223 4.4 19.8 1.0
OG1 B:THR87 4.4 29.2 1.0
O3 B:PRP402 4.7 25.9 1.0
C B:ASP223 4.7 21.0 1.0
OE2 B:GLU224 4.7 22.9 1.0
CB B:GLU224 4.8 13.4 1.0
O B:HOH481 4.8 15.9 1.0
O2B B:PRP402 4.9 20.1 1.0
N B:ASP223 5.0 16.2 1.0

Reference:

S.Schlee, M.Deuss, M.Bruning, A.Ivens, T.Schwab, N.Hellmann, O.Mayans, R.Sterner. Activation of Anthranilate Phosphoribosyltransferase From Sulfolobus Solfataricus By Removal of Magnesium Inhibition and Acceleration of Product Release Biochemistry V. 48 5199 2009.
ISSN: ISSN 0006-2960
PubMed: 19385665
DOI: 10.1021/BI802335S
Page generated: Sat Oct 5 16:22:40 2024

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