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Manganese in PDB 3fyp: Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis

Enzymatic activity of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis

All present enzymatic activity of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis:
2.5.1.55;

Protein crystallography data

The structure of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis, PDB code: 3fyp was solved by G.B.Jameson, E.J.Parker, F.P.Cochrane, M.L.Patchett, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 37.96 / 1.85
Space group P 21 21 21
Cell size a, b, c (Å), α, β, γ (°) 81.781, 85.679, 163.828, 90.00, 90.00, 90.00
R / Rfree (%) 20.5 / 24.7

Other elements in 3fyp:

The structure of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis also contains other interesting chemical elements:

Chlorine (Cl) 2 atoms
Sodium (Na) 2 atoms

Manganese Binding Sites:

The binding sites of Manganese atom in the Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis (pdb code 3fyp). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis, PDB code: 3fyp:

Manganese binding site 1 out of 1 in 3fyp

Go back to Manganese Binding Sites List in 3fyp
Manganese binding site 1 out of 1 in the Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the Quadruple Mutant (N23C/C246S/D247E/P249A) of 3-Deoxy-D-Manno-Octulosonate 8-Phosphate Synthase (KDO8PS) From Neisseria Meningitidis within 5.0Å range:
probe atom residue distance (Å) B Occ
D:Mn284

b:31.0
occ:0.25
 OE1 D:GLU236 2.6 38.8 1.0
NE2 D:HIS199 2.8 28.6 1.0
OE2 D:GLU236 3.0 40.7 1.0
CD D:GLU236 3.2 39.0 1.0
SG D:CYS23 3.3 43.7 1.0
CB D:CYS23 3.7 39.6 1.0
CE1 D:HIS199 3.7 29.2 1.0
CA D:CYS23 3.8 37.1 1.0
CD2 D:HIS199 3.9 31.8 1.0
NZ D:LYS57 4.0 47.4 1.0
O D:HOH555 4.1 53.5 1.0
O2' D:PEP281 4.1 26.0 0.5
C1 D:PEP281 4.5 24.5 0.5
CG D:GLU236 4.6 35.8 1.0
C D:CYS23 4.7 35.0 1.0
O D:CYS23 4.7 30.7 1.0
N D:CYS23 4.9 37.6 1.0
CE D:LYS57 4.9 47.0 1.0
ND1 D:HIS199 4.9 23.7 1.0
NZ D:LYS52 4.9 36.7 1.0
O1 D:PEP281 5.0 23.5 0.5
CG D:HIS199 5.0 26.9 1.0

Reference:

F.C.Cochrane, T.V.Cookson, G.B.Jameson, E.J.Parker. Reversing Evolution: Re-Establishing Obligate Metal Ion Dependence in A Metal-Independent KDO8P Synthase J.Mol.Biol. V. 390 646 2009.
ISSN: ISSN 0022-2836
PubMed: 19447118
DOI: 10.1016/J.JMB.2009.05.014
Page generated: Sat Oct 5 16:20:56 2024

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