Manganese in PDB 3fxo: Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+

All present enzymatic activity of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+:
3.1.3.16;

The structure of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+, PDB code: 3fxo was solved by T.Hu, L.Wang, K.Wang, H.Jiang, X.Shen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	15.00 / 2.50
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	91.115, 91.115, 106.577, 90.00, 90.00, 120.00
R / Rfree (%)	19 / 25.1

The binding sites of Manganese atom in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+ (pdb code 3fxo). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+, PDB code: 3fxo:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn391 b:23.9 occ:1.00 OD2 A:ASP60 2.2 16.2 1.0 OD2 A:ASP282 2.3 19.2 1.0 OD1 A:ASP239 2.3 17.7 1.0 O A:HOH620 2.3 28.1 1.0 O A:HOH554 2.4 13.3 1.0 O A:HOH435 2.5 22.9 1.0 CG A:ASP60 2.9 17.3 1.0 OD1 A:ASP60 3.0 18.4 1.0 CG A:ASP282 3.2 19.0 1.0 CG A:ASP239 3.3 15.2 1.0 OD1 A:ASP282 3.4 19.0 1.0 OD2 A:ASP239 3.6 16.4 1.0 MN A:MN392 3.7 34.1 1.0 O A:HOH553 3.8 17.1 1.0 O2 A:PO4393 4.2 77.7 1.0 N A:GLY240 4.3 14.1 1.0 O A:HOH400 4.3 19.7 1.0 CB A:ASP60 4.3 17.1 1.0 O A:HOH619 4.4 19.9 1.0 O A:HOH402 4.5 28.8 1.0 CB A:ASP282 4.5 17.7 1.0 O A:HOH419 4.6 35.1 1.0 N A:ASP239 4.6 13.6 1.0 O A:ASN283 4.6 16.4 1.0 CB A:ASP239 4.7 14.3 1.0 CB A:CYS238 4.7 13.3 1.0 OD1 A:ASP38 4.8 20.1 1.0 SG A:CYS238 4.8 14.5 1.0 CA A:GLY240 4.9 14.2 1.0 O A:HOH444 4.9 10.0 1.0 C A:ASP239 4.9 14.0 1.0 CA A:ASP239 4.9 14.0 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 1 Mm of MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn392 b:34.1 occ:1.00 O A:HOH554 2.2 13.3 1.0 O A:GLY61 2.2 18.8 1.0 O A:HOH402 2.2 28.8 1.0 OD1 A:ASP60 2.3 18.4 1.0 O A:HOH553 2.5 17.1 1.0 O A:HOH416 2.6 21.6 1.0 C A:GLY61 3.4 18.4 1.0 CG A:ASP60 3.5 17.3 1.0 MN A:MN391 3.7 23.9 1.0 N A:GLY61 3.9 17.7 1.0 O A:HOH444 4.1 10.0 1.0 OD2 A:ASP60 4.1 16.2 1.0 OE1 A:GLU37 4.1 23.1 1.0 O1 A:PO4393 4.1 78.0 1.0 C A:ASP60 4.1 17.5 1.0 O A:HOH620 4.2 28.1 1.0 CA A:GLY61 4.2 18.1 1.0 CB A:GLU37 4.2 23.4 1.0 N A:HIS62 4.3 18.9 1.0 OD1 A:ASP38 4.3 20.1 1.0 CA A:HIS62 4.4 19.5 1.0 O2 A:PO4393 4.5 77.7 1.0 OD1 A:ASN283 4.5 15.4 1.0 O A:HOH435 4.5 22.9 1.0 O A:ASP60 4.5 17.7 1.0 CB A:HIS62 4.6 19.3 1.0 OD1 A:ASP282 4.6 19.0 1.0 CA A:ASP60 4.6 17.1 1.0 CB A:ASP60 4.7 17.1 1.0 O A:HOH460 4.7 24.4 1.0 O A:HOH485 4.8 34.0 1.0 P A:PO4393 4.9 78.0 1.0 O A:GLU37 4.9 22.5 1.0 C A:GLU37 4.9 22.8 1.0

T.Hu, L.Wang, K.Wang, J.Chen, H.Jiang, X.Shen. Structural Basis For the MN2+-Dependent Activation of Human PPM1A To Be Published.