Manganese in PDB 3fxm: Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+

All present enzymatic activity of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+:
3.1.3.16;

The structure of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+, PDB code: 3fxm was solved by T.Hu, L.Wang, K.Wang, H.Jiang, X.Shen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.97 / 2.50
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	91.069, 91.069, 106.508, 90.00, 90.00, 120.00
R / Rfree (%)	18.3 / 25.1

The binding sites of Manganese atom in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+ (pdb code 3fxm). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+, PDB code: 3fxm:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn391 b:19.0 occ:1.00 O A:HOH618 2.1 20.0 1.0 OD1 A:ASP239 2.2 16.9 1.0 O A:HOH435 2.2 26.9 1.0 OD2 A:ASP282 2.2 16.9 1.0 OD2 A:ASP60 2.3 11.9 1.0 O A:HOH553 2.3 2.3 1.0 CG A:ASP60 3.0 14.8 1.0 OD1 A:ASP60 3.1 16.6 1.0 CG A:ASP239 3.2 13.8 1.0 CG A:ASP282 3.2 16.4 1.0 OD2 A:ASP239 3.4 12.5 1.0 OD1 A:ASP282 3.6 16.0 1.0 O A:HOH552 3.6 4.0 1.0 MN A:MN392 3.8 15.6 1.0 N A:GLY240 4.3 14.3 1.0 OB2 A:FLC394 4.3 47.4 1.0 O A:HOH400 4.4 11.9 1.0 CB A:ASP60 4.4 15.1 1.0 O A:HOH617 4.5 20.0 1.0 CB A:ASP282 4.5 15.0 1.0 O A:HOH419 4.5 43.4 1.0 CB A:ASP239 4.5 13.4 1.0 O A:ASN283 4.6 14.5 1.0 OD1 A:ASP38 4.7 16.9 1.0 N A:ASP239 4.7 12.7 1.0 O A:HOH402 4.7 21.1 1.0 CB A:CYS238 4.8 12.6 1.0 C A:ASP239 4.8 13.9 1.0 O A:HOH444 4.8 10.2 1.0 OB1 A:FLC394 4.8 48.5 1.0 SG A:CYS238 4.9 12.9 1.0 CA A:GLY240 4.9 15.2 1.0 CA A:ASP239 4.9 13.4 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Citrate at 10 Mm of MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn392 b:15.6 occ:1.00 O A:HOH416 2.0 14.3 1.0 O A:HOH552 2.1 4.0 1.0 O A:GLY61 2.2 16.0 1.0 O A:HOH402 2.2 21.1 1.0 OD1 A:ASP60 2.2 16.6 1.0 O A:HOH553 2.3 2.3 1.0 C A:GLY61 3.3 16.0 1.0 CG A:ASP60 3.4 14.8 1.0 MN A:MN391 3.8 19.0 1.0 N A:GLY61 3.8 15.3 1.0 OD2 A:ASP60 3.9 11.9 1.0 OA2 A:FLC394 4.0 54.1 1.0 OD1 A:ASP38 4.0 16.9 1.0 OB1 A:FLC394 4.1 48.5 1.0 O A:HOH444 4.1 10.2 1.0 OE1 A:GLU37 4.1 23.4 1.0 C A:ASP60 4.1 15.4 1.0 CA A:GLY61 4.1 15.7 1.0 O A:HOH460 4.2 21.9 1.0 O A:HOH618 4.2 20.0 1.0 O A:HOH435 4.2 26.9 1.0 N A:HIS62 4.3 16.6 1.0 CB A:GLU37 4.3 21.6 1.0 OD1 A:ASN283 4.4 13.1 1.0 CA A:HIS62 4.5 17.0 0.5 CA A:HIS62 4.5 17.1 0.5 CB A:ASP60 4.6 15.1 1.0 CB A:HIS62 4.6 17.1 0.5 CA A:ASP60 4.6 15.1 1.0 OB2 A:FLC394 4.6 47.4 1.0 O A:ASP60 4.6 15.6 1.0 CBC A:FLC394 4.6 50.7 1.0 OD1 A:ASP282 4.7 16.0 1.0 C A:GLU37 4.8 21.1 1.0 O A:GLU37 4.8 21.2 1.0 CAC A:FLC394 5.0 53.3 1.0

T.Hu, L.Wang, K.Wang, J.Chen, H.Jiang, X.Shen. Structural Basis For the MN2+-Dependent Activation of Human PPM1A To Be Published.