Manganese in PDB 3fxj: Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+

All present enzymatic activity of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+:
3.1.3.16;

The structure of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+, PDB code: 3fxj was solved by T.Hu, L.Wang, K.Wang, H.Jiang, X.Shen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	14.97 / 2.50
Space group	P 31 2 1
Cell size a, b, c (Å), α, β, γ (°)	91.382, 91.382, 106.738, 90.00, 90.00, 120.00
R / Rfree (%)	19 / 25.5

The binding sites of Manganese atom in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+ (pdb code 3fxj). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+, PDB code: 3fxj:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn391 b:16.8 occ:1.00 OD1 A:ASP239 2.1 15.1 1.0 OD2 A:ASP282 2.1 12.4 1.0 OD2 A:ASP60 2.2 6.9 1.0 O A:HOH554 2.3 12.1 1.0 O A:HOH435 2.5 13.5 1.0 O A:HOH620 2.6 3.3 1.0 CG A:ASP239 3.0 13.5 1.0 CG A:ASP60 3.0 11.1 1.0 CG A:ASP282 3.1 12.7 1.0 OD1 A:ASP60 3.2 11.3 1.0 OD2 A:ASP239 3.3 14.8 1.0 OD1 A:ASP282 3.5 11.2 1.0 O A:HOH553 3.7 15.2 1.0 MN A:MN392 3.8 20.8 1.0 O2 A:PO4393 3.9 35.1 1.0 O A:HOH619 4.1 7.2 1.0 N A:GLY240 4.3 11.5 1.0 O A:HOH400 4.3 7.7 1.0 CB A:ASP282 4.4 11.4 1.0 CB A:ASP239 4.4 12.0 1.0 CB A:ASP60 4.4 11.7 1.0 O A:ASN283 4.6 11.6 1.0 O A:HOH402 4.6 19.4 1.0 N A:ASP239 4.6 11.1 1.0 O A:HOH419 4.6 12.9 1.0 SG A:CYS238 4.8 12.0 1.0 CA A:ASP239 4.8 11.7 1.0 CB A:CYS238 4.8 10.8 1.0 O3 A:PO4393 4.8 33.4 1.0 OD1 A:ASP38 4.8 11.8 1.0 C A:ASP239 4.9 11.6 1.0 O A:HOH444 4.9 5.7 1.0 P A:PO4393 5.0 33.4 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Human Protein Phosphatase 1A (PPM1A) Bound with Phosphate at 3 Mm of MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn392 b:20.8 occ:1.00 O A:GLY61 2.2 16.4 1.0 O A:HOH402 2.2 19.4 1.0 OD1 A:ASP60 2.2 11.3 1.0 O A:HOH554 2.2 12.1 1.0 O A:HOH553 2.3 15.2 1.0 O A:HOH416 2.5 11.4 1.0 C A:GLY61 3.4 15.5 1.0 CG A:ASP60 3.4 11.1 1.0 MN A:MN391 3.8 16.8 1.0 O2 A:PO4393 3.8 35.1 1.0 N A:GLY61 4.0 13.7 1.0 OD2 A:ASP60 4.0 6.9 1.0 OD1 A:ASP38 4.1 11.8 1.0 C A:ASP60 4.2 13.1 1.0 O A:HOH435 4.2 13.5 1.0 CA A:GLY61 4.2 14.9 1.0 O A:HOH444 4.3 5.7 1.0 O3 A:PO4393 4.3 33.4 1.0 OD1 A:ASN283 4.3 11.3 1.0 N A:HIS62 4.3 16.1 1.0 OE1 A:GLU37 4.3 22.2 1.0 CB A:GLU37 4.4 19.6 1.0 CA A:HIS62 4.4 16.8 0.5 CB A:HIS62 4.5 16.7 0.5 OD1 A:ASP282 4.5 11.2 1.0 CA A:HIS62 4.5 16.5 0.5 P A:PO4393 4.5 33.4 1.0 O A:HOH620 4.6 3.3 1.0 O A:ASP60 4.6 13.6 1.0 CB A:ASP60 4.6 11.7 1.0 CA A:ASP60 4.7 12.0 1.0 O A:HOH460 4.7 18.0 1.0 O1 A:PO4393 4.7 35.0 1.0 O A:GLU37 4.7 19.6 1.0 C A:GLU37 4.8 19.1 1.0 CB A:HIS62 5.0 16.0 0.5 O A:HOH485 5.0 26.9 1.0 OD2 A:ASP282 5.0 12.4 1.0

T.Hu, L.Wang, K.Wang, J.Chen, H.Jiang, X.Shen. Structural Basis For the MN2+-Dependent Activation of Human PPM1A To Be Published.