Manganese in PDB 3aam: Crystal Structure of Endonuclease IV From Thermus Thermophilus HB8

All present enzymatic activity of Crystal Structure of Endonuclease IV From Thermus Thermophilus HB8:
3.1.21.2;

The structure of Crystal Structure of Endonuclease IV From Thermus Thermophilus HB8, PDB code: 3aam was solved by R.Asano, H.Ishikawa, S.Nakane, S.Baba, N.Nakagawa, S.Kuramitsu, R.Masui, Riken Structural Genomics/Proteomics Initiative (Rsgi), with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.12 / 1.58
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	36.646, 85.273, 36.580, 90.00, 105.37, 90.00
R / Rfree (%)	19.9 / 22.3

The binding sites of Manganese atom in the Crystal Structure of Endonuclease IV From Thermus Thermophilus HB8 (pdb code 3aam). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Crystal Structure of Endonuclease IV From Thermus Thermophilus HB8, PDB code: 3aam:

Manganese binding site 1 out of 1 in the Crystal Structure of Endonuclease IV From Thermus Thermophilus HB8


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Endonuclease IV From Thermus Thermophilus HB8 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn271 b:13.3 occ:1.00 O3 A:PO4272 2.0 17.8 1.0 NE2 A:HIS172 2.2 8.3 1.0 NE2 A:HIS219 2.3 8.1 1.0 OD1 A:ASP217 2.4 15.9 1.0 OD2 A:ASP217 2.5 16.8 1.0 O A:HOH333 2.5 18.8 1.0 CG A:ASP217 2.8 16.5 1.0 CE1 A:HIS219 3.2 9.6 1.0 CD2 A:HIS172 3.2 8.4 1.0 CE1 A:HIS172 3.2 8.7 1.0 CD2 A:HIS219 3.3 9.6 1.0 P A:PO4272 3.6 15.7 1.0 O1 A:PO4272 4.1 14.7 1.0 CB A:ASP217 4.3 15.4 1.0 ND1 A:HIS219 4.3 9.5 1.0 ND1 A:HIS172 4.3 8.2 1.0 CG A:HIS172 4.4 6.6 1.0 CG A:HIS219 4.4 9.4 1.0 O4 A:PO4272 4.4 16.9 1.0 OD2 A:ASP169 4.4 8.8 1.0 O2 A:PO4272 4.5 16.9 1.0 O A:HOH280 4.8 10.6 1.0 O A:HOH325 4.9 12.3 1.0 O A:ASP217 5.0 15.0 1.0

R.Asano, H.Ishikawa, S.Nakane, N.Nakagawa, S.Kuramitsu, R.Masui. An Additional C-Terminal Loop in Endonuclease IV, An Apurinic/Apyrimidinic Endonuclease, Controls Binding Affinity to Dna Acta Crystallogr.,Sect.D V. 67 149 2011.
ISSN: ISSN 0907-4449
PubMed: 21358045
DOI: 10.1107/S0907444910052479