Manganese in PDB 2zxp: Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8

The structure of Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8, PDB code: 2zxp was solved by T.Wakamatsu, Y.Kitamura, N.Nakagawa, R.Masui, S.Kuramitsu, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	41.56 / 2.30
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	83.127, 83.127, 249.937, 90.00, 90.00, 90.00
R / Rfree (%)	23.2 / 28.1

The binding sites of Manganese atom in the Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8 (pdb code 2zxp). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8, PDB code: 2zxp:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn667 b:31.5 occ:1.00 OD1 A:ASP221 2.0 28.4 1.0 OD2 A:ASP136 2.1 36.9 1.0 NE2 A:HIS160 2.3 43.0 1.0 OD2 A:ASP84 2.4 19.7 1.0 O A:HOH683 2.5 27.8 1.0 CG A:ASP136 2.9 37.4 1.0 OD1 A:ASP136 3.0 39.5 1.0 CG A:ASP221 3.1 26.5 1.0 O A:HOH761 3.1 36.0 1.0 CD2 A:HIS160 3.2 44.6 1.0 NE2 A:HIS161 3.2 63.1 1.0 CE1 A:HIS160 3.3 43.6 1.0 CG A:ASP84 3.3 18.4 1.0 OD2 A:ASP221 3.5 27.1 1.0 CB A:ASP84 3.6 16.9 1.0 MN A:MN668 3.7 65.2 1.0 CD2 A:HIS161 3.8 62.3 1.0 N A:GLY187 4.1 18.5 1.0 CB A:ASP136 4.2 37.6 1.0 CB A:ASP221 4.3 25.0 1.0 CE1 A:HIS161 4.3 63.7 1.0 CG A:HIS160 4.4 45.1 1.0 ND1 A:HIS160 4.4 44.0 1.0 OD1 A:ASP84 4.4 17.1 1.0 CB A:THR186 4.6 19.0 1.0 CA A:GLY187 4.6 17.5 1.0 OD1 A:ASP82 4.9 36.9 1.0 CA A:ASP136 4.9 38.3 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Recj in Complex with MN2+ From Thermus Thermophilus HB8 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn668 b:65.2 occ:1.00 OD1 A:ASP136 1.9 39.5 1.0 OD1 A:ASP80 2.3 41.5 1.0 OD2 A:ASP82 2.3 35.2 1.0 O A:HOH683 2.5 27.8 1.0 OD1 A:ASP82 2.5 36.9 1.0 CG A:ASP82 2.7 34.3 1.0 CG A:ASP136 3.1 37.4 1.0 CG A:ASP80 3.2 39.6 1.0 CB A:ASP80 3.5 38.3 1.0 OD2 A:ASP136 3.7 36.9 1.0 MN A:MN667 3.7 31.5 1.0 NE2 A:HIS161 3.9 63.1 1.0 CE1 A:HIS161 4.1 63.7 1.0 CB A:ASP82 4.1 30.7 1.0 N A:GLY85 4.2 18.1 1.0 CB A:ASP136 4.2 37.6 1.0 OD2 A:ASP80 4.3 39.4 1.0 O A:ASP136 4.5 41.0 1.0 CB A:ASP84 4.5 16.9 1.0 OD2 A:ASP221 4.6 27.1 1.0 N A:ASP82 4.6 31.4 1.0 O A:HOH899 4.7 24.4 1.0 CA A:GLY85 4.7 16.6 1.0 O A:HOH761 4.7 36.0 1.0 CA A:ASP82 4.9 29.5 1.0 OD2 A:ASP84 4.9 19.7 1.0 OD1 A:ASP221 4.9 28.4 1.0 CA A:ASP80 4.9 37.6 1.0

T.Wakamatsu, Y.Kitamura, Y.Kotera, N.Nakagawa, S.Kuramitsu, R.Masui. Structure of Recj Exonuclease Defines Its Specificity For Single-Stranded Dna J.Biol.Chem. V. 285 9762 2010.
ISSN: ISSN 0021-9258
PubMed: 20129927
DOI: 10.1074/JBC.M109.096487