Manganese in PDB 2wgz: Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal)

All present enzymatic activity of Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal):
2.4.1.151; 2.4.1.87;

The structure of Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal), PDB code: 2wgz was solved by H.Jamaluddin, P.Tumbale, T.A.Ferns, N.Thiyagarajan, K.Brew, K.R.Acharya, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.29 / 2.12
Space group	P 1 21 1
Cell size a, b, c (Å), α, β, γ (°)	45.146, 94.222, 94.465, 90.00, 99.05, 90.00
R / Rfree (%)	16.865 / 20.582

The binding sites of Manganese atom in the Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal) (pdb code 2wgz). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal), PDB code: 2wgz:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1400 b:14.7 occ:1.00 O2B A:UDP1401 2.2 13.9 1.0 OD1 A:ASP227 2.2 12.6 1.0 OD2 A:ASP225 2.2 12.7 1.0 O A:HOH2272 2.2 11.8 1.0 O1A A:UDP1401 2.2 13.3 1.0 OD2 A:ASP227 2.5 13.7 1.0 CG A:ASP227 2.7 13.4 1.0 CG A:ASP225 3.2 12.8 1.0 PB A:UDP1401 3.2 14.9 1.0 PA A:UDP1401 3.5 13.3 1.0 O3A A:UDP1401 3.6 13.1 1.0 CB A:ASP225 3.6 12.9 1.0 O1B A:UDP1401 3.7 14.2 1.0 NZ A:LYS359 3.7 12.5 1.0 O A:HOH2275 3.8 20.5 1.0 O3' A:UDP1401 3.9 12.2 1.0 O A:HOH2256 4.2 13.3 1.0 CB A:ASP227 4.2 13.2 1.0 OD1 A:ASP225 4.3 13.3 1.0 C5' A:UDP1401 4.3 13.2 1.0 O5' A:UDP1401 4.4 12.8 1.0 O A:HOH2155 4.5 13.0 1.0 O2A A:UDP1401 4.5 12.8 1.0 O A:HOH2252 4.5 8.6 1.0 O3B A:UDP1401 4.5 14.4 1.0 O A:HOH2154 4.5 22.7 1.0 O A:HOH2156 4.5 10.1 1.0 C3' A:UDP1401 4.6 12.2 1.0 CB A:ALA282 4.7 11.2 1.0 O A:ASP227 4.8 13.1 1.0 C4' A:UDP1401 4.9 12.4 1.0 N A:ASP227 4.9 13.1 1.0 CA A:ASP227 5.0 13.1 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Alpha-1,3 Galactosyltransferase ( ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnp-Beta-Gal) within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1400 b:14.4 occ:1.00 O2B B:UDP1401 2.2 12.8 1.0 OD1 B:ASP227 2.2 13.3 1.0 OD2 B:ASP225 2.2 10.3 1.0 O1A B:UDP1401 2.3 14.1 1.0 O B:HOH2267 2.3 10.7 1.0 OD2 B:ASP227 2.5 14.8 1.0 CG B:ASP227 2.7 13.8 1.0 CG B:ASP225 3.2 12.5 1.0 PB B:UDP1401 3.3 14.4 1.0 PA B:UDP1401 3.5 14.3 1.0 CB B:ASP225 3.6 12.8 1.0 O3A B:UDP1401 3.7 13.2 1.0 O1B B:UDP1401 3.7 13.8 1.0 NZ B:LYS359 3.7 14.3 1.0 O3' B:UDP1401 3.9 13.0 1.0 O B:HOH2271 3.9 17.7 1.0 O B:HOH2251 4.1 13.0 1.0 CB B:ASP227 4.2 13.0 1.0 C5' B:UDP1401 4.3 13.7 1.0 OD1 B:ASP225 4.3 12.6 1.0 O B:HOH2149 4.4 12.4 1.0 O5' B:UDP1401 4.4 13.7 1.0 O B:HOH2148 4.5 25.4 1.0 O B:HOH2247 4.5 9.2 1.0 O2A B:UDP1401 4.5 13.8 1.0 O B:HOH2150 4.6 9.7 1.0 O3B B:UDP1401 4.6 13.6 1.0 C3' B:UDP1401 4.7 12.9 1.0 CB B:ALA282 4.7 11.0 1.0 O B:ASP227 4.8 13.3 1.0 C4' B:UDP1401 4.9 12.9 1.0 N B:ASP227 4.9 12.9 1.0 CA B:ASP227 5.0 13.3 1.0

H.Jamaluddin, P.Tumbale, T.A.Ferns, N.Thiyagarajan, K.Brew, K.R.Acharya. Crystal Structure of Alpha-1,3 Galactosyltransferase (ALPHA3GT) in A Complex with P-Nitrophenyl-Beta-Galactoside (Pnpbetagal). Biochem.Biophys.Res.Commun. V. 385 601 2009.
ISSN: ISSN 0006-291X
PubMed: 19486884
DOI: 10.1016/J.BBRC.2009.05.111