Manganese in PDB 2vic: Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese

The structure of Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese, PDB code: 2vic was solved by O.Barabas, D.R.Ronning, C.Guynet, A.B.Hickman, B.Ton-Hoang, M.Chandler, F.Dyda, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.47 / 2.35
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	74.206, 50.261, 115.625, 90.00, 105.60, 90.00
R / Rfree (%)	20.3 / 24.1

The binding sites of Manganese atom in the Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese (pdb code 2vic). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese, PDB code: 2vic:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1156 b:0.6 occ:1.00 NE2 A:HIS66 2.6 30.4 1.0 OE1 B:GLN131 3.3 40.0 1.0 CE1 A:HIS66 3.5 30.3 1.0 CD2 A:HIS66 3.7 30.1 1.0 CG A:HIS64 3.8 44.7 1.0 CB A:HIS64 4.0 42.4 1.0 ND1 A:HIS64 4.0 46.2 1.0 CD2 A:HIS64 4.1 45.9 1.0 OD1 A:ASP61 4.2 62.5 1.0 CD B:GLN131 4.3 37.2 1.0 CB A:ASP61 4.4 52.2 1.0 CE1 A:HIS64 4.5 46.5 1.0 CG A:ASP61 4.5 58.3 1.0 NE2 A:HIS64 4.5 46.3 1.0 OH B:TYR127 4.6 67.0 1.0 ND1 A:HIS66 4.7 30.0 1.0 CG A:HIS66 4.8 29.3 1.0 CG B:GLN131 4.9 41.7 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of the ISHP608 Transposase in Complex with Left End 26-Mer Dna and Manganese within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn1042 b:57.8 occ:1.00 O D:HOH2033 2.2 52.0 1.0 O D:HOH2034 2.2 53.5 1.0 O D:HOH2031 2.2 54.5 1.0 O D:HOH2035 2.2 52.8 1.0 O D:HOH2032 2.2 49.3 1.0 O6 D:DG39 2.7 43.8 1.0 C6 D:DG39 3.6 34.9 1.0 N7 D:DG39 3.7 31.7 1.0 OD1 A:ASN12 3.9 25.7 1.0 N4 D:DC22 3.9 27.5 1.0 OP1 D:DG19 3.9 21.6 1.0 C5 D:DG39 4.0 32.8 1.0 O A:HIS11 4.1 31.3 1.0 O6 D:DG40 4.1 26.9 1.0 O6 D:DG38 4.3 19.9 1.0 O D:HOH2006 4.4 31.8 1.0 O D:HOH2010 4.4 20.3 1.0 O D:HOH2012 4.4 41.4 1.0 ND2 B:ASN109 4.6 22.8 1.0 N4 D:DC21 4.6 19.7 1.0 CG A:ASN12 4.8 18.4 1.0 N1 D:DG39 4.9 36.7 1.0 CB A:HIS11 4.9 32.8 1.0 C8 D:DG39 4.9 29.0 1.0 C A:HIS11 4.9 31.4 1.0 C6 D:DG40 5.0 23.8 1.0

O.Barabas, D.R.Ronning, C.Guynet, A.B.Hickman, B.Ton-Hoang, M.Chandler, F.Dyda. Mechanism of IS200/IS605 Family Dna Transposases: Activation and Transposon-Directed Target Site Selection. Cell(Cambridge,Mass.) V. 132 208 2008.
ISSN: ISSN 0092-8674
PubMed: 18243097
DOI: 10.1016/J.CELL.2007.12.029