Manganese in PDB 2v8j: Structure of A Family 2 Pectate Lyase in Complex with A Transition Metal

All present enzymatic activity of Structure of A Family 2 Pectate Lyase in Complex with A Transition Metal:
4.2.2.2;

The structure of Structure of A Family 2 Pectate Lyase in Complex with A Transition Metal, PDB code: 2v8j was solved by D.W.Abbott, A.B.Boraston, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.01 / 2.01
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	68.180, 78.010, 104.200, 90.00, 90.00, 90.00
R / Rfree (%)	20.3 / 24.7

The binding sites of Manganese atom in the Structure of A Family 2 Pectate Lyase in Complex with A Transition Metal (pdb code 2v8j). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the Structure of A Family 2 Pectate Lyase in Complex with A Transition Metal, PDB code: 2v8j:

Manganese binding site 1 out of 1 in the Structure of A Family 2 Pectate Lyase in Complex with A Transition Metal


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Structure of A Family 2 Pectate Lyase in Complex with A Transition Metal within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1540 b:18.5 occ:1.00 O A:HOH2344 2.0 20.3 1.0 NE2 A:HIS109 2.1 23.4 1.0 O A:HOH2345 2.1 26.2 1.0 O A:HOH2346 2.1 31.3 1.0 OE1 A:GLU130 2.2 22.1 1.0 NE2 A:HIS172 2.2 22.3 1.0 CE1 A:HIS109 3.1 25.0 1.0 CD2 A:HIS109 3.1 23.9 1.0 CD2 A:HIS172 3.1 22.2 1.0 CD A:GLU130 3.2 21.8 1.0 CE1 A:HIS172 3.2 24.6 1.0 OE2 A:GLU130 3.5 25.6 1.0 OE1 A:GLU515 4.1 29.8 1.0 OE2 A:GLU126 4.2 29.4 1.0 ND1 A:HIS109 4.2 23.6 1.0 CG A:HIS109 4.2 24.4 1.0 ND1 A:HIS172 4.3 24.3 1.0 CG A:HIS172 4.3 23.4 1.0 CG A:GLU130 4.5 21.1 1.0 CD A:GLU126 4.7 27.2 1.0 CB A:GLU130 4.8 20.5 1.0 OE1 A:GLU126 4.9 28.4 1.0

D.W.Abbott, A.B.Boraston. A Family 2 Pectate Lyase Displays A Rare Fold and Transition Metal-Assisted -Elimination. J.Biol.Chem. V. 282 35328 2007.
ISSN: ISSN 0021-9258
PubMed: 17881361
DOI: 10.1074/JBC.M705511200