Manganese in PDB 2v09: SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc

All present enzymatic activity of SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc:
4.1.1.2;

The structure of SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc, PDB code: 2v09 was solved by M.R.Burrell, V.J.Just, L.Bowater, S.A.Fairhurst, L.Requena, D.M.Lawson, S.Bornemann, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.22 / 1.8
Space group	H 3 2
Cell size a, b, c (Å), α, β, γ (°)	154.602, 154.602, 122.957, 90.00, 90.00, 120.00
R / Rfree (%)	14.1 / 17.3

The binding sites of Manganese atom in the SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc (pdb code 2v09). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc, PDB code: 2v09:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1383 b:9.2 occ:1.00 OE1 A:GLU101 2.1 6.9 1.0 NE2 A:HIS95 2.2 8.4 1.0 NE2 A:HIS97 2.3 7.0 1.0 NE2 A:HIS140 2.3 5.9 1.0 O A:HOH2467 2.3 11.7 1.0 O A:HOH2466 2.4 10.7 1.0 CD A:GLU101 3.1 8.8 1.0 CE1 A:HIS97 3.2 8.2 1.0 CE1 A:HIS95 3.2 7.8 1.0 CD2 A:HIS95 3.2 9.2 1.0 CE1 A:HIS140 3.2 8.6 1.0 CD2 A:HIS140 3.2 7.2 1.0 CD2 A:HIS97 3.3 11.4 1.0 O A:HOH2465 3.5 28.5 1.0 OE2 A:GLU101 3.5 10.0 1.0 O A:HOH2241 4.3 30.9 1.0 ND1 A:HIS97 4.3 8.9 1.0 ND1 A:HIS95 4.3 8.6 1.0 ND1 A:HIS140 4.3 8.4 1.0 CG A:HIS95 4.4 8.9 1.0 CG A:HIS140 4.4 7.0 1.0 CE1 A:PHE155 4.4 11.7 1.0 CG A:HIS97 4.4 7.1 1.0 CG A:GLU101 4.5 8.4 1.0 CB A:GLU101 4.7 8.0 1.0 O A:HOH2159 4.8 18.5 1.0 CZ A:PHE155 4.9 11.0 1.0

Manganese binding site 2 out of 2 in the SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of SENS161-164DSSN Mutant of Bacillus Subtilis Oxalate Decarboxylase Oxdc within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1384 b:10.8 occ:1.00 OE1 A:GLU280 2.1 12.2 1.0 NE2 A:HIS275 2.2 6.3 1.0 O A:HOH2468 2.2 9.3 1.0 NE2 A:HIS273 2.2 8.9 1.0 NE2 A:HIS319 2.3 8.9 1.0 CD A:GLU280 3.1 10.2 1.0 CD2 A:HIS275 3.1 6.9 1.0 CE1 A:HIS273 3.1 7.3 1.0 CE1 A:HIS319 3.2 9.3 1.0 CE1 A:HIS275 3.2 5.6 1.0 CD2 A:HIS273 3.2 9.4 1.0 CD2 A:HIS319 3.3 8.1 1.0 OE2 A:GLU280 3.4 11.3 1.0 OE2 A:GLU333 3.5 17.7 1.0 CD A:GLU333 4.0 15.4 1.0 CG A:HIS275 4.3 8.3 1.0 ND1 A:HIS273 4.3 9.2 1.0 ND1 A:HIS275 4.3 7.3 1.0 CG A:HIS273 4.3 7.6 1.0 ND1 A:HIS319 4.3 9.3 1.0 CG A:HIS319 4.4 8.8 1.0 CG A:GLU280 4.4 9.1 1.0 NH2 A:ARG270 4.5 14.2 1.0 CG A:GLU333 4.5 10.8 1.0 OE1 A:GLU333 4.6 23.2 1.0 CZ A:PHE335 4.7 12.3 1.0 CB A:GLU280 4.8 8.7 1.0 NE A:ARG270 4.9 15.0 1.0 CZ A:ARG270 5.0 15.4 1.0

M.R.Burrell, V.J.Just, L.Bowater, S.A.Fairhurst, L.Requena, D.M.Lawson, S.Bornemann. Oxalate Decarboxylase and Oxalate Oxidase Activities Can Be Interchanged with A Specificity Switch of Up to 282 000 By Mutating An Active Site Lid. Biochemistry V. 46 12327 2007.
ISSN: ISSN 0006-2960
PubMed: 17924657
DOI: 10.1021/BI700947S