Manganese in PDB 2rl9: Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside

The structure of Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside, PDB code: 2rl9 was solved by L.J.Olson, O.Hindsgaul, N.M.Dahms, J.-J.P.Kim, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 2.40
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	92.628, 92.628, 85.594, 90.00, 90.00, 90.00
R / Rfree (%)	19.9 / 26.5

The binding sites of Manganese atom in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside (pdb code 2rl9). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside, PDB code: 2rl9:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3000 b:39.9 occ:1.00 OD2 A:ASP103 2.4 30.7 1.0 O2P A:M6P3001 2.4 26.2 1.0 O3P A:M6P3001 3.1 23.9 1.0 P A:M6P3001 3.2 23.6 1.0 CG A:ASP103 3.2 26.9 1.0 C6 A:M6P3001 3.5 25.7 1.0 O3 A:MAN3003 3.6 44.2 1.0 CB A:ASP103 3.7 23.7 1.0 O6 A:M6P3001 3.9 25.5 1.0 NH2 A:ARG111 4.0 11.6 1.0 N A:ASP103 4.0 20.8 1.0 OD1 A:ASP103 4.2 27.9 1.0 O A:HOH3024 4.2 32.4 1.0 O5 A:M6P3001 4.4 26.6 1.0 CA A:ASP103 4.5 23.9 1.0 O1P A:M6P3001 4.6 26.1 1.0 C5 A:M6P3001 4.7 23.4 1.0 O A:GLU101 4.7 18.4 1.0 C A:TYR102 4.8 20.1 1.0 C3 A:MAN3003 4.9 43.4 1.0 NH1 A:ARG111 4.9 12.4 1.0 CZ A:ARG111 4.9 13.0 1.0 CA A:TYR102 5.0 20.0 1.0

Manganese binding site 2 out of 2 in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to Trimannoside within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn3001 b:54.6 occ:1.00 OD2 B:ASP103 2.3 35.6 1.0 O2P B:M6P3004 2.6 26.5 1.0 O3P B:M6P3004 3.3 26.6 1.0 CG B:ASP103 3.4 34.2 1.0 P B:M6P3004 3.5 25.7 1.0 C6 B:M6P3004 3.6 29.2 1.0 CB B:ASP103 3.9 30.1 1.0 O3 B:MAN3003 4.0 46.0 1.0 O6 B:M6P3004 4.2 25.8 1.0 NH2 B:ARG111 4.4 24.9 1.0 O5 B:M6P3004 4.4 34.8 1.0 N B:ASP103 4.5 27.3 1.0 OD1 B:ASP103 4.5 35.5 1.0 C5 B:M6P3004 4.7 31.9 1.0 CA B:ASP103 4.8 28.7 1.0 O1P B:M6P3004 4.9 27.2 1.0

L.J.Olson, O.Hindsgaul, N.M.Dahms, J.J.Kim. Structural Insights Into the Mechanism of pH-Dependent Ligand Binding and Release By the Cation-Dependent Mannose 6-Phosphate Receptor. J.Biol.Chem. V. 283 10124 2008.
ISSN: ISSN 0021-9258
PubMed: 18272523
DOI: 10.1074/JBC.M708994200