Manganese in PDB 2rl8: Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P

The structure of Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P, PDB code: 2rl8 was solved by L.J.Olson, O.Hindsgaul, J.-J.P.Kim, N.M.Dahms, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	30.00 / 1.45
Space group	P 43 21 2
Cell size a, b, c (Å), α, β, γ (°)	91.659, 91.659, 85.079, 90.00, 90.00, 90.00
R / Rfree (%)	21.9 / 23.7

The binding sites of Manganese atom in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P (pdb code 2rl8). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P, PDB code: 2rl8:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn503 b:23.1 occ:1.00 OD2 A:ASP103 2.2 25.6 1.0 O3P A:M6D500 2.2 14.1 1.0 O A:HOH662 2.3 28.4 1.0 O A:HOH586 2.3 24.3 1.0 O A:HOH706 2.4 27.0 1.0 CG A:ASP103 3.2 23.4 1.0 P A:M6D500 3.5 13.2 1.0 CB A:ASP103 3.5 19.4 1.0 O2P A:M6D500 3.7 17.1 1.0 O A:HOH808 3.9 40.9 1.0 N A:ASP103 4.0 15.3 1.0 O6 A:M6D500 4.1 13.0 1.0 O A:HOH888 4.3 37.5 1.0 OD1 A:ASP103 4.4 24.5 1.0 CA A:ASP103 4.4 17.6 1.0 NH2 A:ARG111 4.5 11.3 1.0 O5 A:M6D500 4.6 12.5 1.0 O1P A:M6D500 4.6 15.6 1.0 O A:GLU101 4.7 13.4 1.0 O A:HOH552 4.7 18.8 1.0 C A:TYR102 4.7 13.8 1.0 C6 A:M6D500 4.8 12.8 1.0 CA A:TYR102 5.0 13.5 1.0

Manganese binding site 2 out of 2 in the Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure Cation-Dependent Mannose 6-Phosphate Receptor at pH 6.5 Bound to M6P within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn502 b:20.0 occ:1.00 O3P B:M6D501 2.2 14.3 1.0 OD2 B:ASP103 2.2 22.1 1.0 O B:HOH585 2.3 22.6 1.0 O B:HOH649 2.3 25.3 1.0 CG B:ASP103 3.3 20.8 1.0 P B:M6D501 3.4 15.0 1.0 O2P B:M6D501 3.6 18.0 1.0 CB B:ASP103 3.6 18.5 1.0 O6 B:M6D501 4.0 16.7 1.0 O B:HOH639 4.2 24.0 1.0 N B:ASP103 4.3 15.2 1.0 O B:HOH622 4.4 25.2 1.0 O5 B:M6D501 4.4 14.9 1.0 OD1 B:ASP103 4.4 21.7 1.0 O B:HOH901 4.5 38.4 1.0 NH2 B:ARG111 4.5 13.5 1.0 O1P B:M6D501 4.6 16.9 1.0 CA B:ASP103 4.6 16.7 1.0 C6 B:M6D501 4.7 16.3 1.0

L.J.Olson, O.Hindsgaul, N.M.Dahms, J.J.Kim. Structural Insights Into the Mechanism of pH-Dependent Ligand Binding and Release By the Cation-Dependent Mannose 6-Phosphate Receptor. J.Biol.Chem. V. 283 10124 2008.
ISSN: ISSN 0021-9258
PubMed: 18272523
DOI: 10.1074/JBC.M708994200