Manganese in PDB 2rj9: B-Specific Alpha-1,3-Galactosyltransferase (Gtb) + Udp+ Amino-Deoxy- Acceptor

All present enzymatic activity of B-Specific Alpha-1,3-Galactosyltransferase (Gtb) + Udp+ Amino-Deoxy- Acceptor:
2.4.1.37;

The structure of B-Specific Alpha-1,3-Galactosyltransferase (Gtb) + Udp+ Amino-Deoxy- Acceptor, PDB code: 2rj9 was solved by S.V.Evans, J.A.Alfaro, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	19.83 / 1.69
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	52.430, 149.930, 78.920, 90.00, 90.00, 90.00
R / Rfree (%)	20 / 23.2

The binding sites of Manganese atom in the B-Specific Alpha-1,3-Galactosyltransferase (Gtb) + Udp+ Amino-Deoxy- Acceptor (pdb code 2rj9). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the B-Specific Alpha-1,3-Galactosyltransferase (Gtb) + Udp+ Amino-Deoxy- Acceptor, PDB code: 2rj9:

Manganese binding site 1 out of 1 in the B-Specific Alpha-1,3-Galactosyltransferase (Gtb) + Udp+ Amino-Deoxy- Acceptor


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of B-Specific Alpha-1,3-Galactosyltransferase (Gtb) + Udp+ Amino-Deoxy- Acceptor within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1 b:23.9 occ:1.00 O1A A:UDP357 2.2 10.9 0.5 O2B A:UDP357 2.2 16.5 0.5 O A:HOH535 2.2 24.5 1.0 OD2 A:ASP211 2.2 20.9 1.0 OD1 A:ASP213 2.4 17.1 1.0 OD2 A:ASP213 2.6 22.2 1.0 CG A:ASP213 2.8 16.0 1.0 O3A A:UDP357 2.9 14.3 0.5 PB A:UDP357 3.2 14.8 0.5 CG A:ASP211 3.3 17.8 1.0 PA A:UDP357 3.5 13.4 0.5 CB A:ASP211 3.6 16.0 1.0 O3' A:UDP357 3.8 19.5 1.0 O1B A:UDP357 4.1 23.9 1.0 C5' A:UDP357 4.2 17.4 1.0 NZ A:LYS346 4.3 31.2 1.0 CB A:ASP213 4.3 18.4 1.0 OD1 A:ASP211 4.4 19.3 1.0 O5' A:UDP357 4.4 10.9 0.5 O3B A:UDP357 4.4 16.3 0.5 O A:HOH419 4.4 31.7 1.0 C3' A:UDP357 4.6 19.1 1.0 O2A A:UDP357 4.7 12.5 0.5 O A:HOH436 4.7 24.9 1.0 SD A:MET214 4.9 22.2 1.0 O A:ASP213 4.9 17.5 1.0 C4' A:UDP357 4.9 19.2 1.0 CE A:LYS346 4.9 33.1 1.0

J.A.Alfaro, R.B.Zheng, M.Persson, J.A.Letts, R.Polakowski, Y.Bai, S.N.Borisova, N.O.Seto, T.L.Lowary, M.M.Palcic, S.V.Evans. Abo(H) Blood Group A and B Glycosyltransferases Recognize Substrate Via Specific Conformational Changes. J.Biol.Chem. V. 283 10097 2008.
ISSN: ISSN 0021-9258
PubMed: 18192272
DOI: 10.1074/JBC.M708669200