Manganese in PDB 2rj8: B-Specific Alpha-1,3-Galactosyltransferase (Gtb) +Udp+ H-Antigen Disaccharide

All present enzymatic activity of B-Specific Alpha-1,3-Galactosyltransferase (Gtb) +Udp+ H-Antigen Disaccharide:
2.4.1.37;

The structure of B-Specific Alpha-1,3-Galactosyltransferase (Gtb) +Udp+ H-Antigen Disaccharide, PDB code: 2rj8 was solved by S.V.Evans, J.A.Alfaro, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.69
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	52.450, 149.600, 78.740, 90.00, 90.00, 90.00
R / Rfree (%)	19.8 / 22.2

The binding sites of Manganese atom in the B-Specific Alpha-1,3-Galactosyltransferase (Gtb) +Udp+ H-Antigen Disaccharide (pdb code 2rj8). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the B-Specific Alpha-1,3-Galactosyltransferase (Gtb) +Udp+ H-Antigen Disaccharide, PDB code: 2rj8:

Manganese binding site 1 out of 1 in the B-Specific Alpha-1,3-Galactosyltransferase (Gtb) +Udp+ H-Antigen Disaccharide


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of B-Specific Alpha-1,3-Galactosyltransferase (Gtb) +Udp+ H-Antigen Disaccharide within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn356 b:18.9 occ:1.00 OD2 A:ASP211 2.1 16.3 1.0 O3B A:UDP1 2.1 21.8 1.0 O1A A:UDP1 2.2 17.9 1.0 OD1 A:ASP213 2.3 15.8 1.0 OD2 A:ASP213 2.6 20.3 1.0 CG A:ASP213 2.8 17.0 1.0 CG A:ASP211 3.1 15.9 1.0 PB A:UDP1 3.3 19.8 1.0 PA A:UDP1 3.4 20.3 1.0 O3A A:UDP1 3.6 18.9 1.0 CB A:ASP211 3.6 15.7 1.0 O3' A:UDP1 3.8 16.7 1.0 O A:HOH630 3.8 39.3 1.0 O1B A:UDP1 4.0 21.5 1.0 C5' A:UDP1 4.2 17.0 1.0 OD1 A:ASP211 4.2 17.4 1.0 NZ A:LYS346 4.3 22.0 1.0 CB A:ASP213 4.3 16.8 1.0 O5' A:UDP1 4.3 18.3 1.0 O A:HOH522 4.5 24.9 1.0 O2A A:UDP1 4.5 19.9 1.0 C3' A:UDP1 4.6 16.6 1.0 O2B A:UDP1 4.6 20.4 1.0 O A:HOH493 4.8 22.0 1.0 C4' A:UDP1 4.8 17.3 1.0 O A:HOH502 4.9 21.4 1.0 SD A:MET214 4.9 20.6 1.0 CB A:ALA268 4.9 13.6 1.0 O A:ASP213 4.9 16.1 1.0 N A:ASP213 5.0 15.0 1.0

J.A.Alfaro, R.B.Zheng, M.Persson, J.A.Letts, R.Polakowski, Y.Bai, S.N.Borisova, N.O.Seto, T.L.Lowary, M.M.Palcic, S.V.Evans. Abo(H) Blood Group A and B Glycosyltransferases Recognize Substrate Via Specific Conformational Changes. J.Biol.Chem. V. 283 10097 2008.
ISSN: ISSN 0021-9258
PubMed: 18192272
DOI: 10.1074/JBC.M708669200