Manganese in PDB 2rj5: B-Specific Alpha-1,3-Galactosyltransferase G176R S235G Mutant (Aabb) +Udp

All present enzymatic activity of B-Specific Alpha-1,3-Galactosyltransferase G176R S235G Mutant (Aabb) +Udp:
2.4.1.37;

The structure of B-Specific Alpha-1,3-Galactosyltransferase G176R S235G Mutant (Aabb) +Udp, PDB code: 2rj5 was solved by S.V.Evans, J.A.Alfaro, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	20.00 / 1.45
Space group	C 2 2 21
Cell size a, b, c (Å), α, β, γ (°)	52.590, 149.020, 79.650, 90.00, 90.00, 90.00
R / Rfree (%)	20.3 / 21.7

The binding sites of Manganese atom in the B-Specific Alpha-1,3-Galactosyltransferase G176R S235G Mutant (Aabb) +Udp (pdb code 2rj5). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total only one binding site of Manganese was determined in the B-Specific Alpha-1,3-Galactosyltransferase G176R S235G Mutant (Aabb) +Udp, PDB code: 2rj5:

Manganese binding site 1 out of 1 in the B-Specific Alpha-1,3-Galactosyltransferase G176R S235G Mutant (Aabb) +Udp


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of B-Specific Alpha-1,3-Galactosyltransferase G176R S235G Mutant (Aabb) +Udp within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2 b:12.8 occ:0.50 O3B A:UDP1 2.1 15.9 0.5 O1A A:UDP1 2.2 13.2 0.5 O A:HOH524 2.3 27.8 1.0 OD1 A:ASP213 2.4 13.9 1.0 OD2 A:ASP211 2.4 15.9 1.0 OD2 A:ASP213 2.6 19.1 1.0 CG A:ASP213 2.9 13.8 1.0 PB A:UDP1 3.3 16.0 0.5 PA A:UDP1 3.4 13.8 0.5 CG A:ASP211 3.4 13.2 1.0 O3A A:UDP1 3.5 14.2 0.5 CB A:ASP211 3.6 10.5 1.0 O3' A:UDP1 3.7 18.5 1.0 O1B A:UDP1 4.1 16.4 0.5 C5' A:UDP1 4.3 17.3 1.0 O5' A:UDP1 4.4 14.0 0.5 CB A:ASP213 4.4 11.9 1.0 O2A A:UDP1 4.5 13.5 0.5 C3' A:UDP1 4.5 16.2 0.5 OD1 A:ASP211 4.5 14.8 1.0 O2B A:UDP1 4.5 16.3 0.5 C4' A:UDP1 4.8 17.0 1.0 O A:ASP213 5.0 10.7 1.0

J.A.Alfaro, R.B.Zheng, M.Persson, J.A.Letts, R.Polakowski, Y.Bai, S.N.Borisova, N.O.Seto, T.L.Lowary, M.M.Palcic, S.V.Evans. Abo(H) Blood Group A and B Glycosyltransferases Recognize Substrate Via Specific Conformational Changes. J.Biol.Chem. V. 283 10097 2008.
ISSN: ISSN 0021-9258
PubMed: 18192272
DOI: 10.1074/JBC.M708669200