Manganese in PDB 2phk: The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition

All present enzymatic activity of The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition:
2.7.1.38;

The structure of The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition, PDB code: 2phk was solved by E.D.Lowe, M.E.M.Noble, V.T.Skamnaki, N.G.Oikonomakos, D.J.Owen, L.N.Johnson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	25.00 / 2.60
Space group	P 32 2 1
Cell size a, b, c (Å), α, β, γ (°)	65.300, 65.300, 145.800, 90.00, 90.00, 120.00
R / Rfree (%)	23.6 / 30

The binding sites of Manganese atom in the The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition (pdb code 2phk). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition, PDB code: 2phk:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn382 b:9.6 occ:1.00 O3G A:ATP381 2.3 13.9 1.0 O2B A:ATP381 2.3 13.1 1.0 O A:HOH418 2.3 18.8 1.0 OD2 A:ASP167 2.3 14.1 1.0 OD1 A:ASP167 2.4 14.6 1.0 O A:HOH405 2.5 4.0 1.0 CG A:ASP167 2.7 14.6 1.0 PG A:ATP381 3.3 13.4 1.0 PB A:ATP381 3.4 13.4 1.0 O3B A:ATP381 3.6 13.3 1.0 O2G A:ATP381 3.7 12.7 1.0 MN A:MN383 3.9 10.5 1.0 OD2 A:ASP149 3.9 13.3 1.0 OG B:SER5 4.0 45.3 1.0 O A:HOH392 4.2 8.2 1.0 CB A:ASP167 4.2 14.2 1.0 O1B A:ATP381 4.3 12.8 1.0 O3A A:ATP381 4.5 14.1 1.0 CA A:GLY169 4.6 12.3 1.0 NZ A:LYS48 4.6 13.6 1.0 O1G A:ATP381 4.6 12.8 1.0 O2A A:ATP381 4.6 14.2 1.0 O A:HOH394 4.6 7.1 1.0 N A:GLY169 4.7 11.7 1.0 CB B:SER5 4.9 45.8 1.0 PA A:ATP381 4.9 14.6 1.0 O1A A:ATP381 5.0 14.8 1.0

Manganese binding site 2 out of 2 in the The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn383 b:10.5 occ:1.00 O2G A:ATP381 2.0 12.7 1.0 O2A A:ATP381 2.1 14.2 1.0 OD2 A:ASP167 2.2 14.1 1.0 O A:HOH438 2.2 31.3 1.0 OD1 A:ASN154 2.3 12.8 1.0 O3B A:ATP381 2.7 13.3 1.0 PG A:ATP381 2.8 13.4 1.0 CG A:ASN154 3.2 13.8 1.0 CG A:ASP167 3.2 14.6 1.0 PA A:ATP381 3.4 14.6 1.0 ND2 A:ASN154 3.5 13.2 1.0 O3G A:ATP381 3.6 13.9 1.0 PB A:ATP381 3.7 13.4 1.0 O3A A:ATP381 3.7 14.1 1.0 CB A:ASP167 3.8 14.2 1.0 O2B A:ATP381 3.8 13.1 1.0 MN A:MN382 3.9 9.6 1.0 O1G A:ATP381 4.1 12.8 1.0 OD1 A:ASP167 4.3 14.6 1.0 O1A A:ATP381 4.3 14.8 1.0 CE A:LYS151 4.3 10.6 1.0 O5' A:ATP381 4.4 16.2 1.0 C5' A:ATP381 4.6 17.4 1.0 NZ A:LYS151 4.6 10.3 1.0 O3' A:ATP381 4.6 18.0 1.0 CB A:ASN154 4.6 14.1 1.0 O B:HOH49 4.7 28.9 1.0 OD2 A:ASP149 4.7 13.3 1.0 O A:GLU153 4.8 13.2 1.0 C3' A:ATP381 5.0 18.4 1.0

E.D.Lowe, M.E.Noble, V.T.Skamnaki, N.G.Oikonomakos, D.J.Owen, L.N.Johnson. The Crystal Structure of A Phosphorylase Kinase Peptide Substrate Complex: Kinase Substrate Recognition. Embo J. V. 16 6646 1997.
ISSN: ISSN 0261-4189
PubMed: 9362479
DOI: 10.1093/EMBOJ/16.22.6646