Manganese in PDB 2o8g: Rat PP1C Gamma Complexed with Mouse Inhibitor-2

All present enzymatic activity of Rat PP1C Gamma Complexed with Mouse Inhibitor-2:
3.1.3.16;

The structure of Rat PP1C Gamma Complexed with Mouse Inhibitor-2, PDB code: 2o8g was solved by T.D.Hurley, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	48.80 / 2.50
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	96.267, 103.234, 149.992, 90.00, 90.00, 90.00
R / Rfree (%)	19.4 / 23.5

The binding sites of Manganese atom in the Rat PP1C Gamma Complexed with Mouse Inhibitor-2 (pdb code 2o8g). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Rat PP1C Gamma Complexed with Mouse Inhibitor-2, PDB code: 2o8g:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Rat PP1C Gamma Complexed with Mouse Inhibitor-2


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Rat PP1C Gamma Complexed with Mouse Inhibitor-2 within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn324 b:51.8 occ:1.00 OD1 A:ASN124 2.2 32.9 1.0 NE2 A:HIS173 2.3 36.2 1.0 OD1 A:ASP92 2.3 35.5 1.0 O A:HOH360 2.4 33.8 1.0 ND1 A:HIS248 2.5 30.3 1.0 CG A:ASP92 3.1 35.1 1.0 CD2 A:HIS173 3.2 34.6 1.0 CE1 A:HIS173 3.2 35.1 1.0 OD2 A:ASP92 3.2 35.9 1.0 CG A:ASN124 3.3 33.4 1.0 CE1 A:HIS248 3.3 28.9 1.0 CG A:HIS248 3.6 30.8 1.0 O I:HOH208 3.7 29.0 1.0 CA A:HIS248 3.7 31.6 1.0 ND2 A:ASN124 3.9 32.5 1.0 OD2 A:ASP64 4.0 38.9 1.0 CB A:HIS248 4.0 31.0 1.0 O A:HIS248 4.0 32.2 1.0 N A:ASN124 4.3 34.4 1.0 CD2 A:HIS125 4.3 32.6 1.0 ND1 A:HIS173 4.3 34.0 1.0 C A:HIS248 4.4 31.9 1.0 CG A:HIS173 4.4 34.1 1.0 CB A:ASN124 4.4 34.0 1.0 CB A:ASP92 4.5 34.1 1.0 O A:HOH325 4.5 37.1 1.0 NE2 A:HIS248 4.5 28.7 1.0 N A:HIS248 4.6 31.5 1.0 CD2 A:HIS248 4.7 30.6 1.0 O A:LEU205 4.7 33.8 1.0 CA A:ASN124 4.8 34.0 1.0 CG A:ASP64 4.9 37.2 1.0 OD1 A:ASP64 4.9 37.5 1.0

Manganese binding site 2 out of 2 in the Rat PP1C Gamma Complexed with Mouse Inhibitor-2


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Rat PP1C Gamma Complexed with Mouse Inhibitor-2 within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn324 b:52.1 occ:1.00 OD1 B:ASN124 2.3 32.8 1.0 NE2 B:HIS173 2.3 34.7 1.0 OD1 B:ASP92 2.4 35.1 1.0 O B:HOH367 2.4 32.7 1.0 ND1 B:HIS248 2.4 32.1 1.0 CE1 B:HIS248 3.2 31.1 1.0 CD2 B:HIS173 3.2 35.4 1.0 CG B:ASP92 3.3 35.2 1.0 CG B:ASN124 3.3 32.8 1.0 CE1 B:HIS173 3.3 34.1 1.0 OD2 B:ASP92 3.4 35.3 1.0 CG B:HIS248 3.5 31.3 1.0 O J:HOH210 3.6 26.1 1.0 CA B:HIS248 3.6 32.2 1.0 O B:HIS248 3.9 32.5 1.0 ND2 B:ASN124 3.9 29.9 1.0 CB B:HIS248 3.9 31.7 1.0 OD2 B:ASP64 3.9 42.0 1.0 C B:HIS248 4.2 32.8 1.0 N B:ASN124 4.3 33.8 1.0 CD2 B:HIS125 4.4 36.4 1.0 NE2 B:HIS248 4.4 32.3 1.0 CG B:HIS173 4.4 36.3 1.0 ND1 B:HIS173 4.4 35.2 1.0 O B:HOH325 4.4 37.0 1.0 CB B:ASN124 4.5 33.2 1.0 CD2 B:HIS248 4.5 31.6 1.0 CB B:ASP92 4.6 35.5 1.0 N B:HIS248 4.6 32.5 1.0 O B:LEU205 4.7 34.8 1.0 CG B:ASP64 4.8 40.9 1.0 OD1 B:ASP64 4.8 40.9 1.0 CA B:ASN124 4.9 33.6 1.0

T.D.Hurley, J.Yang, L.Zhang, K.D.Goodwin, Q.Zou, M.Cortese, A.K.Dunker, A.A.Depaoli-Roach. Structural Basis For Regulation of Protein Phosphatase 1 By Inhibitor-2. J.Biol.Chem. V. 282 28874 2007.
ISSN: ISSN 0021-9258
PubMed: 17636256
DOI: 10.1074/JBC.M703472200