Manganese in PDB 2ltn: Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum)

The structure of Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum), PDB code: 2ltn was solved by F.L.Suddath, S.R.Phillips, H.Einspahr, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	N/A / 1.70
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	50.730, 61.160, 136.590, 90.00, 90.00, 90.00
R / Rfree (%)	n/a / n/a

The structure of Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum) also contains other interesting chemical elements:

Calcium

(Ca)

2 atoms

The binding sites of Manganese atom in the Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum) (pdb code 2ltn). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum), PDB code: 2ltn:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum) within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn190 b:10.5 occ:1.00 OD2 A:ASP121 2.1 9.7 1.0 O A:HOH456 2.1 12.0 1.0 OD1 A:ASP129 2.2 8.8 1.0 OE1 A:GLU119 2.2 8.0 1.0 NE2 A:HIS136 2.2 9.1 1.0 O A:HOH422 2.3 9.3 1.0 CG A:ASP121 3.1 5.5 1.0 CG A:ASP129 3.1 9.1 1.0 CD2 A:HIS136 3.1 9.2 1.0 CE1 A:HIS136 3.2 9.6 1.0 CD A:GLU119 3.3 9.9 1.0 CB A:ASP121 3.5 6.7 1.0 OE2 A:GLU119 3.6 10.7 1.0 OD2 A:ASP129 3.7 7.4 1.0 O A:HOH657 3.9 7.7 1.0 OD1 A:ASP121 4.2 8.9 1.0 CA A:CA191 4.2 9.2 1.0 OG A:SER146 4.3 9.6 1.0 CB A:ASP129 4.3 8.8 1.0 CG A:HIS136 4.3 8.9 1.0 ND1 A:HIS136 4.3 9.5 1.0 O A:HOH480 4.4 15.8 1.0 O A:ILE144 4.5 8.8 1.0 CG A:GLU119 4.6 8.2 1.0 CA A:ASP129 4.7 10.6 1.0 CD A:PRO130 4.7 13.3 1.0 CD1 A:TRP128 4.7 11.5 1.0 NE1 A:TRP128 4.7 11.7 1.0

Manganese binding site 2 out of 2 in the Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum)


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum) within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn190 b:11.4 occ:1.00 OD2 C:ASP121 1.9 10.2 1.0 O C:HOH455 2.0 12.8 1.0 NE2 C:HIS136 2.2 11.4 1.0 O C:HOH458 2.2 12.4 1.0 OE1 C:GLU119 2.2 8.8 1.0 OD1 C:ASP129 2.2 9.1 1.0 CG C:ASP121 3.1 5.4 1.0 CE1 C:HIS136 3.1 9.9 1.0 CD C:GLU119 3.1 9.5 1.0 CD2 C:HIS136 3.2 10.3 1.0 CG C:ASP129 3.2 10.8 1.0 CB C:ASP121 3.5 8.1 1.0 OE2 C:GLU119 3.6 10.4 1.0 OD2 C:ASP129 3.8 11.8 1.0 O C:HOH457 3.9 15.7 1.0 CA C:CA191 4.2 10.5 1.0 OD1 C:ASP121 4.2 9.4 1.0 ND1 C:HIS136 4.3 11.6 1.0 OG C:SER146 4.3 9.8 1.0 CG C:HIS136 4.3 11.2 1.0 CB C:ASP129 4.3 10.4 1.0 O C:HOH414 4.4 16.7 1.0 CG C:GLU119 4.5 8.5 1.0 O C:ILE144 4.5 9.7 1.0 CD C:PRO130 4.6 15.2 1.0 CA C:ASP129 4.7 12.2 1.0 CD1 C:TRP128 4.7 14.2 1.0 NE1 C:TRP128 4.7 13.6 1.0

T.Prasthofer, S.R.Phillips, F.L.Suddath, J.A.Engler. Design, Expression, and Crystallization of Recombinant Lectin From the Garden Pea (Pisum Sativum). J.Biol.Chem. V. 264 6793 1989.
ISSN: ISSN 0021-9258
PubMed: 2708344