Manganese in the structure of Klenow Fragment With Normal Substrate And Zinc and Manganese (pdb 2kzm)

The binding sites of Manganese atom in the structure of Klenow Fragment With Normal Substrate And Zinc and Manganese (pdb code 2kzm). This binding sites where shown with 5.0 Angstroms radius around Manganese atom. The 2kzm structure was solved by C.A.BRAUTIGAM, S.SUN, J.A.PICCIRILLI, T.A.STEITZ, with X-Ray Crystallography technique, brief refinement statistics is given in the table below: Resolution (A) 20.0-2.6 Space group P43 a (A) 102.600 b (A) 102.600 c (A) 86.200 alpha (°) 90.00 beta (°) 90.00 gamma (°) 90.00 Rfactor (%) 19.5 Rfree (%) 24.3 Manganese Binding Sites: Manganese binding site 1 out of 1 in 2kzm Click to enlarge Click to enlarge Mono- and Stereo- picture of 5.0 Angstrom coordination sphere 1 of Manganese in the PDB 2kzm. Coordination sphere was calculated for all residues within 5.0 Angstroms distance from the central Manganese atom, shown by VdW sphere Residues shown as a stick model or VDW spheres: B: Dg1007, B: Dc1008, A: Asp355, A: Thr356, A: Gln419, A: Tyr423, A: Asp424, A: Asp501, A: Zn1, A: Hoh18, conact list: Atom Atom Distance (A) Mn C3' B:Dg1007 3.78 Mn O3' B:Dg1007 2.63 Mn O4' B:Dg1007 4.77 Mn C5' B:Dg1007 4.45 Mn C2' B:Dg1007 4.48 Mn C4' B:Dg1007 3.73 Mn OP1 B:Dc1008 2.42 Mn P B:Dc1008 3.10 Mn OP2 B:Dc1008 4.29 Mn C5' B:Dc1008 3.93 Mn O5' B:Dc1008 4.00 Mn CB A:Asp355 4.60 Mn OD2 A:Asp355 3.50 Mn OD1 A:Asp355 2.32 Mn CG A:Asp355 3.25 Mn O A:Thr356 4.03 Mn N A:Thr356 4.71 Mn O A:Gln419 4.40 Mn CD2 A:Tyr423 4.84 Mn OD2 A:Asp424 4.22 Mn OD1 A:Asp424 4.06 Mn CG A:Asp424 4.57 Mn OD2 A:Asp501 4.86 Mn ZN A:Zn1 3.65 Mn O A:Hoh18 4.62 interactive model: