Manganese in PDB 2jlf: Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase

All present enzymatic activity of Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase:
2.7.7.48;

The structure of Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase, PDB code: 2jlf was solved by M.M.Poranen, P.S.Salgado, M.R.L.Koivunen, S.Wright, D.H.Bamford, D.I.Stuart, J.M.Grimes, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	18.021 / 3.2
Space group	P 32
Cell size a, b, c (Å), α, β, γ (°)	110.040, 110.040, 159.132, 90.00, 90.00, 120.00
R / Rfree (%)	22.75 / 28.6

The binding sites of Manganese atom in the Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase (pdb code 2jlf). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase, PDB code: 2jlf:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn1664 b:59.3 occ:1.00 OD1 A:ASP454 2.1 42.2 1.0 OE1 A:GLN491 2.4 52.9 1.0 O A:ALA495 2.5 22.9 1.0 CD A:GLN491 2.8 48.0 1.0 NE2 A:GLN491 2.9 55.0 1.0 CG A:ASP454 3.3 41.2 1.0 O A:THR323 3.6 43.1 1.0 C A:ALA495 3.7 20.8 1.0 OD2 A:ASP454 3.8 39.3 1.0 O A:ALA455 4.0 23.1 1.0 CG A:GLN491 4.0 51.3 1.0 N A:ALA495 4.3 21.6 1.0 CB A:GLN491 4.3 30.0 1.0 N A:ALA455 4.4 37.8 1.0 CB A:ALA322 4.4 22.3 1.0 CA A:PHE496 4.5 21.2 1.0 N A:PHE496 4.5 17.8 1.0 CB A:ASP454 4.6 33.3 1.0 CA A:ALA495 4.6 20.9 1.0 CA A:ASP454 4.7 40.5 1.0 C A:THR323 4.7 30.6 1.0 C A:ALA455 4.8 30.5 1.0 N A:THR323 4.9 22.3 1.0 CA A:ALA322 5.0 20.3 1.0 C A:ALA322 5.0 21.3 1.0

Manganese binding site 2 out of 2 in the Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Structural Explanation For the Role of Mn in the Activity of PHI6 Rna-Dependent Rna Polymerase within 5.0Å range: probe atom residue distance (Å) B Occ B:Mn1664 b:67.3 occ:1.00 OE1 B:GLN491 2.2 42.5 1.0 OD1 B:ASP454 2.4 35.6 1.0 CD B:GLN491 2.5 50.6 1.0 NE2 B:GLN491 2.6 61.4 1.0 O B:ALA495 2.7 27.2 1.0 O B:THR323 3.3 36.3 1.0 CG B:ASP454 3.6 34.8 1.0 CG B:GLN491 3.7 49.3 1.0 C B:ALA495 3.9 21.5 1.0 CB B:GLN491 4.0 42.7 1.0 OD2 B:ASP454 4.1 32.8 1.0 O B:ALA455 4.2 22.7 1.0 N B:ALA495 4.3 31.0 1.0 CB B:ALA322 4.3 19.5 1.0 C B:THR323 4.5 30.6 1.0 N B:ALA455 4.6 36.3 1.0 CA B:ALA495 4.7 20.3 1.0 N B:THR323 4.7 24.1 1.0 CB B:ASP454 4.8 32.2 1.0 N B:PHE496 4.8 21.1 1.0 C B:ALA322 4.8 20.7 1.0 CA B:ASP454 4.8 37.3 1.0 CA B:PHE496 4.9 24.7 1.0 CA B:ALA322 4.9 22.6 1.0

M.M.Poranen, P.S.Salgado, M.R.L.Koivunen, S.Wright, D.H.Bamford, D.I.Stuart, J.M.Grimes. Structural Explanation For the Role of MN2+ in the Activity of {Phi}6 Rna-Dependent Rna Polymerase. Nucleic Acids Res. V. 36 6633 2008.
ISSN: ISSN 0305-1048
PubMed: 18940872
DOI: 10.1093/NAR/GKN632