Manganese in PDB 2jck: Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion

All present enzymatic activity of Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion:
2.4.1.151; 2.4.1.87;

The structure of Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion, PDB code: 2jck was solved by H.Jamaluddin, P.Tumbale, S.G.Withers, K.R.Acharya, K.Brew, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	24.84 / 1.80
Space group	C 1 2 1
Cell size a, b, c (Å), α, β, γ (°)	122.824, 68.251, 44.732, 90.00, 90.19, 90.00
R / Rfree (%)	18.2 / 19.2

The binding sites of Manganese atom in the Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion (pdb code 2jck). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion, PDB code: 2jck:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3362 b:13.7 occ:1.00 OD1 A:ASP1227 2.2 10.8 1.0 OD2 A:ASP1225 2.2 12.7 1.0 O1B A:UDP3361 2.3 14.5 1.0 O1A A:UDP3361 2.3 12.4 1.0 O A:HOH2406 2.4 23.2 1.0 OD2 A:ASP1227 2.5 12.2 1.0 CG A:ASP1227 2.7 11.7 1.0 CG A:ASP1225 3.2 12.7 1.0 PB A:UDP3361 3.2 15.4 1.0 PA A:UDP3361 3.4 14.2 1.0 O3A A:UDP3361 3.5 13.2 1.0 CB A:ASP1225 3.5 12.2 1.0 O3' A:UDP3361 3.8 11.2 1.0 O3B A:UDP3361 3.8 15.2 1.0 O A:HOH2324 4.1 23.9 1.0 CB A:ASP1227 4.1 11.8 1.0 O2B A:UDP3361 4.2 14.9 1.0 OD1 A:ASP1225 4.3 12.7 1.0 O A:HOH2259 4.3 22.4 1.0 C5' A:UDP3361 4.3 12.5 1.0 O2A A:UDP3361 4.3 13.4 1.0 O5' A:UDP3361 4.4 12.6 1.0 O A:HOH2258 4.4 28.0 1.0 O A:ASP1227 4.6 12.5 1.0 C3' A:UDP3361 4.6 12.7 1.0 O A:HOH2260 4.7 23.5 1.0 N A:ASP1227 4.9 11.6 1.0 C4' A:UDP3361 4.9 12.6 1.0 O A:HOH2409 4.9 28.2 1.0 CA A:ASP1225 4.9 11.3 1.0 CA A:ASP1227 4.9 12.1 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Alpha-1,3 Galactosyltransferase (R365K) in Complex with Udp and 2 Manganese Ion within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3363 b:58.5 occ:0.90 O A:HOH2407 2.8 31.9 1.0 OE2 A:GLU1317 3.0 11.0 1.0 OE1 A:GLU1317 3.0 11.1 1.0 O A:HOH2409 3.2 28.2 1.0 OE1 A:GLN1247 3.2 16.1 1.0 O A:HOH2408 3.2 40.6 1.0 CD A:GLU1317 3.4 11.7 1.0 CE1 A:HIS1280 3.6 9.5 1.0 ND1 A:HIS1280 3.6 12.2 1.0 O A:HOH2363 3.8 26.8 0.7 CG A:GLN1247 3.9 12.2 1.0 CD A:GLN1247 4.0 15.0 1.0 O A:HOH2324 4.4 23.9 1.0 O A:HOH2085 4.5 37.6 1.0 NE2 A:HIS1280 4.5 11.1 1.0 CG A:HIS1280 4.6 10.6 1.0 O A:HOH2288 4.7 37.8 1.0 CE1 A:TYR1278 4.8 12.7 1.0 CH2 A:TRP1356 4.9 15.5 1.0 CG A:GLU1317 4.9 10.5 1.0

H.Jamaluddin, P.Tumbale, S.G.Withers, K.R.Acharya, K.Brew. Conformational Changes Induced By Binding Udp-2F- Galactose to Alpha-1,3 Galactosyltransferase- Implications For Catalysis. J.Mol.Biol. V. 369 1270 2007.
ISSN: ISSN 0022-2836
PubMed: 17493636
DOI: 10.1016/J.JMB.2007.04.012