Manganese in PDB 2im3: Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+

All present enzymatic activity of Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+:
2.7.7.48;

The structure of Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+, PDB code: 2im3 was solved by A.A.Thompson, O.B.Peersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	29.70 / 2.60
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	128.115, 128.115, 112.833, 90.00, 90.00, 120.00
R / Rfree (%)	23.7 / 26.9

The structure of Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+ also contains other interesting chemical elements:

Arsenic	(As)	4 atoms
Sodium	(Na)	1 atom

The binding sites of Manganese atom in the Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+ (pdb code 2im3). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+, PDB code: 2im3:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2001 b:0.8 occ:1.00 O2B A:UTP1001 2.7 0.6 1.0 O A:TYR234 2.8 40.2 1.0 OD2 A:ASP328 3.1 41.4 1.0 O A:HOH584 3.4 56.2 1.0 O3G A:UTP1001 3.4 0.7 1.0 O1B A:UTP1001 3.5 0.4 1.0 PB A:UTP1001 3.6 0.4 1.0 O A:HOH533 3.6 36.3 1.0 O A:HOH583 3.7 47.2 1.0 MN A:MN2002 3.8 0.5 1.0 C A:TYR234 4.0 39.1 1.0 PG A:UTP1001 4.1 0.4 1.0 O1G A:UTP1001 4.2 0.2 1.0 CG A:ASP328 4.3 40.2 1.0 O3B A:UTP1001 4.4 0.3 1.0 N A:TYR234 4.6 42.9 1.0 CB A:ASP233 4.7 55.0 1.0 O2A A:UTP1001 4.8 0.2 1.0 CA A:TYR234 4.9 39.0 1.0 N A:THR235 5.0 38.6 1.0 CA A:THR235 5.0 36.7 1.0 O3A A:UTP1001 5.0 0.3 1.0

Manganese binding site 2 out of 2 in the Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Poliovirus Polymerase Complexed with Utp and MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2002 b:0.5 occ:1.00 OD2 A:ASP328 2.5 41.4 1.0 OD1 A:ASP328 2.8 45.3 1.0 CG A:ASP328 2.9 40.2 1.0 O A:HOH584 3.3 56.2 1.0 O A:HOH533 3.4 36.3 1.0 MN A:MN2001 3.8 0.8 1.0 CB A:ASP329 4.1 39.3 1.0 N A:ASP329 4.1 35.7 1.0 OD2 A:ASP329 4.1 45.9 1.0 CG A:ASP329 4.2 43.0 1.0 CB A:ASP328 4.4 36.8 1.0 CA A:ASP329 4.4 36.1 1.0 C A:ASP328 4.6 35.5 1.0 OD1 A:ASP329 4.8 46.8 1.0 O A:HOH583 5.0 47.2 1.0 CA A:ASP328 5.0 34.6 1.0

A.A.Thompson, R.A.Albertini, O.B.Peersen. Stabilization of Poliovirus Polymerase By Ntp Binding and Fingers-Thumb Interactions. J.Mol.Biol. V. 366 1459 2007.
ISSN: ISSN 0022-2836
PubMed: 17223130
DOI: 10.1016/J.JMB.2006.11.070