Manganese in PDB 2im1: Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+

All present enzymatic activity of Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+:
2.7.7.48;

The structure of Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+, PDB code: 2im1 was solved by A.A.Thompson, O.B.Peersen, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	28.20 / 2.50
Space group	P 65
Cell size a, b, c (Å), α, β, γ (°)	128.942, 128.942, 112.979, 90.00, 90.00, 120.00
R / Rfree (%)	23.9 / 26.8

The structure of Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+ also contains other interesting chemical elements:

Arsenic	(As)	4 atoms
Sodium	(Na)	1 atom

The binding sites of Manganese atom in the Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+ (pdb code 2im1). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 3 binding sites of Manganese where determined in the Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+, PDB code: 2im1:
Jump to Manganese binding site number: 1; 2; 3;

Manganese binding site 1 out of 3 in the Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2001 b:0.4 occ:1.00 OD2 A:ASP328 3.1 42.8 1.0 MN A:MN2002 3.7 85.3 1.0 O A:HOH549 3.7 33.3 1.0 CG A:ASP328 4.0 39.9 1.0 OD2 A:ASP329 4.1 43.4 1.0 OD1 A:ASP328 4.3 43.5 1.0 CG A:ASP329 4.6 40.9 1.0 CB A:ASP329 4.7 38.6 1.0 N A:ASP329 4.7 37.1 1.0 CA A:ASP329 4.8 35.5 1.0 CB A:ASP233 4.9 45.8 1.0

Manganese binding site 2 out of 3 in the Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2002 b:85.3 occ:1.00 O2B A:CTP1001 2.3 88.4 1.0 O3G A:CTP1001 2.9 93.1 1.0 O A:HOH549 3.2 33.3 1.0 O A:TYR234 3.2 34.8 1.0 PB A:CTP1001 3.3 88.6 1.0 O3B A:CTP1001 3.4 91.5 1.0 PG A:CTP1001 3.6 93.3 1.0 MN A:MN2001 3.7 0.4 1.0 O1A A:CTP1001 3.8 91.9 1.0 OD2 A:ASP328 4.2 42.8 1.0 O1B A:CTP1001 4.3 90.5 1.0 MN A:MN2003 4.3 0.9 1.0 O2G A:CTP1001 4.5 94.4 1.0 C A:TYR234 4.5 35.0 1.0 CB A:ASP233 4.5 45.8 1.0 O3A A:CTP1001 4.6 90.4 1.0 PA A:CTP1001 4.9 89.9 1.0

Manganese binding site 3 out of 3 in the Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Crystal Structure of Poliovirus Polymerase Complexed with Ctp and MN2+ within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn2003 b:0.9 occ:1.00 NZ A:LYS167 2.1 69.4 1.0 O3B A:CTP1001 2.5 91.5 1.0 O1A A:CTP1001 2.7 91.9 1.0 O2G A:CTP1001 2.8 94.4 1.0 PG A:CTP1001 3.3 93.3 1.0 PA A:CTP1001 3.4 89.9 1.0 O A:HOH549 3.4 33.3 1.0 O A:HOH617 3.5 46.9 1.0 CE A:LYS167 3.6 67.3 1.0 PB A:CTP1001 3.7 88.6 1.0 NZ A:LYS359 3.8 72.8 1.0 O3A A:CTP1001 4.0 90.4 1.0 O2A A:CTP1001 4.0 91.0 1.0 CE A:LYS359 4.2 71.9 1.0 O3G A:CTP1001 4.2 93.1 1.0 O2B A:CTP1001 4.3 88.4 1.0 MN A:MN2002 4.3 85.3 1.0 O1G A:CTP1001 4.5 93.1 1.0 CD A:LYS167 4.6 65.6 1.0 O5' A:CTP1001 4.8 89.2 1.0

A.A.Thompson, R.A.Albertini, O.B.Peersen. Stabilization of Poliovirus Polymerase By Ntp Binding and Fingers-Thumb Interactions. J.Mol.Biol. V. 366 1459 2007.
ISSN: ISSN 0022-2836
PubMed: 17223130
DOI: 10.1016/J.JMB.2006.11.070