Manganese in PDB 2iie: Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna

The structure of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna, PDB code: 2iie was solved by Q.Bao, P.Droege, C.A.Davey, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	40.00 / 2.41
Space group	P 21 21 21
Cell size a, b, c (Å), α, β, γ (°)	47.371, 55.311, 177.833, 90.00, 90.00, 90.00
R / Rfree (%)	23.3 / 27.2

The binding sites of Manganese atom in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna (pdb code 2iie). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 7 binding sites of Manganese where determined in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna, PDB code: 2iie:
Jump to Manganese binding site number: 1; 2; 3; 4; 5; 6; 7;

Manganese binding site 1 out of 7 in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ C:Mn307 b:60.9 occ:0.61 O4' C:DG-18 3.5 63.6 1.0 C4' C:DG-18 3.7 66.5 1.0 C5' C:DG-18 4.0 67.5 1.0 O2 C:DT-19 4.3 60.1 1.0 C1' C:DG-18 4.7 60.8 1.0 N3 C:DG-18 5.0 48.1 1.0

Manganese binding site 2 out of 7 in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn303 b:52.3 occ:1.00 O D:HOH946 2.3 41.1 1.0 OP2 D:DT28 2.3 32.6 1.0 O D:HOH934 2.6 48.2 1.0 P D:DT28 3.6 34.8 1.0 C5' D:DT28 4.1 37.8 1.0 O5' D:DT28 4.2 35.6 1.0 OP1 D:DT28 4.3 37.6 1.0 OP2 D:DT29 4.4 36.1 1.0 C3' D:DT28 4.4 36.6 1.0 C4' D:DT28 4.7 38.5 1.0 O3' D:DA27 4.7 39.2 1.0 OP2 D:DA27 4.7 47.6 1.0 O5' D:DA27 4.8 45.2 1.0 O D:HOH815 5.0 39.1 1.0 O3' D:DT28 5.0 35.7 1.0

Manganese binding site 3 out of 7 in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 3 of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ D:Mn304 b:71.4 occ:0.87 O D:HOH948 2.6 51.7 1.0 N7 D:DA24 2.7 39.5 1.0 C8 D:DA24 3.6 39.0 1.0 C5 D:DA24 3.7 39.1 1.0 N6 D:DA24 3.9 39.0 1.0 O D:HOH848 4.1 26.1 1.0 C6 D:DA24 4.2 38.6 1.0 O C:HOH852 4.4 24.9 1.0 O6 D:DG25 4.5 36.7 1.0 N7 D:DA23 4.7 38.2 1.0 N9 D:DA24 4.8 38.8 1.0 O D:HOH883 4.9 42.3 1.0 C4 D:DA24 4.9 39.8 1.0 C8 D:DA23 4.9 38.4 1.0

Manganese binding site 4 out of 7 in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 4 of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ E:Mn302 b:50.9 occ:1.00 O E:HOH768 2.3 30.4 1.0 O E:HOH813 2.4 44.8 1.0 O E:HOH945 2.5 52.6 1.0 N7 E:DG30 2.5 39.1 1.0 C8 E:DG30 3.3 40.5 1.0 C5 E:DG30 3.6 36.9 1.0 O6 E:DG30 3.9 34.6 1.0 O E:HOH933 4.0 48.9 1.0 C6 E:DG30 4.2 35.6 1.0 O E:HOH866 4.4 48.3 1.0 O D:HOH710 4.6 43.2 1.0 N9 E:DG30 4.6 41.0 1.0 C4 E:DG30 4.8 38.2 1.0 N4 E:DC31 5.0 34.4 1.0

Manganese binding site 5 out of 7 in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 5 of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn301 b:37.7 occ:1.00 NE2 A:HIS155 2.1 24.0 1.0 O A:HOH789 2.2 28.8 1.0 O C:HOH719 2.3 21.4 1.0 O A:HOH904 2.3 21.7 1.0 O A:HOH716 2.3 23.9 1.0 CD2 A:HIS155 3.0 23.2 1.0 CE1 A:HIS155 3.1 24.4 1.0 OG A:SER153 3.7 32.5 1.0 O C:HOH879 3.9 41.9 1.0 CG A:HIS155 4.2 27.4 1.0 ND1 A:HIS155 4.2 25.1 1.0 ND1 A:HIS180 4.3 27.9 1.0 O C:HOH701 4.4 33.7 1.0 OP2 C:DA-40 4.4 44.2 1.0 O E:HOH787 4.5 32.8 1.0 O A:HOH737 4.6 38.3 1.0 CE A:LYS182 4.6 49.9 1.0 CD A:LYS182 4.8 47.4 1.0 CG A:HIS180 4.8 29.2 1.0 O E:HOH892 4.8 45.3 1.0 CE1 A:HIS180 4.9 27.5 1.0 CB A:SER153 5.0 31.1 1.0 CB A:HIS180 5.0 28.6 1.0

Manganese binding site 6 out of 7 in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 6 of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn305 b:89.4 occ:0.95 OD1 A:ASN84 2.3 52.3 1.0 O A:HOH942 2.5 69.6 1.0 O A:HOH943 2.5 42.9 1.0 CG A:ASN84 3.4 52.9 1.0 O A:HOH704 3.5 35.8 1.0 ND2 A:ASN84 4.2 53.7 1.0 CB A:ASN84 4.2 50.4 1.0 CA A:ASN84 4.5 49.2 1.0

Manganese binding site 7 out of 7 in the Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 7 of Single Chain Integration Host Factor Protein (SCIHF2) in Complex with Dna within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn306 b:87.1 occ:0.82 OD2 A:ASP58 2.4 62.5 1.0 OD1 A:ASP58 2.4 63.8 1.0 OE2 A:GLU54 2.7 63.8 1.0 CG A:ASP58 2.7 61.8 1.0 O A:HOH756 2.9 44.5 1.0 CD A:GLU54 3.6 64.0 1.0 CG A:GLU54 4.0 60.4 1.0 CB A:ASP58 4.1 60.5 1.0 OE1 A:GLU54 4.5 65.2 1.0 CD2 A:PHE57 4.6 59.7 1.0 O A:GLU54 4.7 54.0 1.0 O A:HOH886 4.7 68.9 1.0 CA A:ASP58 4.8 59.8 1.0 O A:HOH809 4.8 59.2 1.0 N A:ASP58 5.0 59.1 1.0

Q.Bao, H.Chen, Y.Liu, J.Yan, P.Droge, C.A.Davey. A Divalent Metal-Mediated Switch Controlling Protein-Induced Dna Bending J.Mol.Biol. V. 367 731 2007.
ISSN: ISSN 0022-2836
PubMed: 17276457
DOI: 10.1016/J.JMB.2006.09.082