Manganese in PDB 2gui: Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III

All present enzymatic activity of Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III:
2.7.7.7;

The structure of Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III, PDB code: 2gui was solved by A.Y.Park, P.D.Carr, D.L.Ollis, N.E.Dixon, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å)	26.88 / 1.60
Space group	P 41 21 2
Cell size a, b, c (Å), α, β, γ (°)	60.099, 60.099, 109.164, 90.00, 90.00, 90.00
R / Rfree (%)	16 / 19.7

The binding sites of Manganese atom in the Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III (pdb code 2gui). This binding sites where shown within 5.0 Angstroms radius around Manganese atom.
In total 2 binding sites of Manganese where determined in the Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III, PDB code: 2gui:
Jump to Manganese binding site number: 1; 2;

Manganese binding site 1 out of 2 in the Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 1 of Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3000 b:7.9 occ:1.00 OD1 A:ASP12 2.0 6.4 1.0 O A:HOH5024 2.1 10.5 1.0 O A:HOH5002 2.2 7.6 1.0 O1P A:U5P2000 2.2 12.5 1.0 O A:HOH5012 2.2 12.1 1.0 O3P A:U5P2000 2.4 10.9 1.0 P A:U5P2000 2.9 12.6 1.0 CG A:ASP12 3.1 7.3 1.0 OD2 A:ASP12 3.5 8.3 1.0 MN A:MN3001 3.7 8.8 1.0 O5' A:U5P2000 4.0 11.4 1.0 C5' A:U5P2000 4.0 11.6 1.0 O A:HOH5003 4.0 7.4 1.0 O2P A:U5P2000 4.0 15.3 1.0 O A:THR13 4.1 7.1 1.0 OD2 A:ASP103 4.1 8.7 1.0 OD1 A:ASP103 4.2 7.8 1.0 C4' A:U5P2100 4.3 11.1 0.5 O A:HOH5016 4.3 10.5 1.0 O A:HIS98 4.4 8.8 1.0 CB A:ASP12 4.4 6.9 1.0 O4' A:U5P2100 4.4 14.4 0.5 O2P A:U5P2100 4.5 9.9 0.5 CG A:ASP103 4.6 7.4 1.0 N A:THR13 4.6 6.6 1.0 C5' A:U5P2100 4.6 11.3 0.5 O5' A:U5P2100 4.8 10.4 0.5 CA A:ASP12 4.8 6.5 1.0 OD2 A:ASP167 4.8 9.8 1.0 O A:HOH5100 5.0 23.7 1.0

Manganese binding site 2 out of 2 in the Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III


Mono view


Stereo pair view

A full contact list of Manganese with other atoms in the Mn binding site number 2 of Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit of E. Coli Dna Polymerase III within 5.0Å range: probe atom residue distance (Å) B Occ A:Mn3001 b:8.8 occ:1.00 O3P A:U5P2000 1.9 10.9 1.0 OE2 A:GLU14 2.0 11.4 1.0 OD2 A:ASP167 2.0 9.8 1.0 OD2 A:ASP12 2.1 8.3 1.0 O A:HOH5100 2.3 23.7 1.0 CD A:GLU14 3.0 9.4 1.0 CG A:ASP167 3.1 11.1 1.0 CG A:ASP12 3.1 7.3 1.0 P A:U5P2000 3.3 12.6 1.0 OE1 A:GLU14 3.3 8.8 1.0 OD1 A:ASP12 3.5 6.4 1.0 CB A:ASP167 3.6 8.6 1.0 MN A:MN3000 3.7 7.9 1.0 O A:THR13 3.7 7.1 1.0 O2P A:U5P2000 3.9 15.3 1.0 O A:GLY163 3.9 9.7 1.0 CB A:HIS162 4.1 16.4 1.0 OD1 A:ASP167 4.1 12.6 1.0 O5' A:U5P2000 4.1 11.4 1.0 O A:HOH5012 4.1 12.1 1.0 C A:GLY163 4.2 9.5 1.0 CG A:GLU14 4.3 7.7 1.0 O1P A:U5P2000 4.3 12.5 1.0 O A:HOH5002 4.4 7.6 1.0 CB A:ASP12 4.4 6.9 1.0 C5' A:U5P2000 4.5 11.6 1.0 CA A:ALA164 4.5 7.9 1.0 NH2 A:ARG159 4.5 14.6 1.0 ND1 A:HIS162 4.5 18.1 1.0 N A:GLY163 4.5 12.1 1.0 N A:ALA164 4.5 8.3 1.0 C A:THR13 4.6 6.8 1.0 CG A:HIS162 4.7 17.7 1.0 C A:HIS162 4.8 14.5 1.0 CA A:GLY163 4.8 10.4 1.0 CA A:HIS162 4.9 15.9 1.0

A.Y.Park, P.D.Carr, D.L.Oliis, N.E.Dixon. Structure and Function of Cyclized Versions of the Proofreading Exonuclease Subunit E. Coli Dna Polymerase III To Be Published.